Difference between revisions of "Cytochrome c"
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{{MitoPedia | {{MitoPedia | ||
|abbr=''c'' | |abbr=''c'' | ||
|description='''Cytochrome ''c''''' is a component of the electron transport | |description='''Cytochrome ''c''''' is a component of the electron transport system ([[ETS]]) in mitochondria. It is a small heme protein loosely associated with the outside side of the inner mitochondrial membrane. The heme group of '''cytochrome ''c''''' transfers electrons from [[complex III]] to the [[complex IV]]. The release of '''cytochrome ''c''''' into the cytoplasm is associated with apoptosis. [http://en.wikipedia.org/wiki/Cytochrome_c]. | ||
|info=[[MiPNet09.12]]; [[Gnaiger_2002_BiochemSocTrans]] | |info=[[MiPNet09.12]]; [[Gnaiger_2002_BiochemSocTrans]] | ||
|type=Substrate ETS | |type=Substrate ETS |
Revision as of 11:50, 3 December 2010
Description
Cytochrome c is a component of the electron transport system (ETS) in mitochondria. It is a small heme protein loosely associated with the outside side of the inner mitochondrial membrane. The heme group of cytochrome c transfers electrons from complex III to the complex IV. The release of cytochrome c into the cytoplasm is associated with apoptosis. [1].
Abbreviation: c
Reference: MiPNet09.12; Gnaiger_2002_BiochemSocTrans
Labels:
Application in HRR
c: Cytochrome c (Sigma C 7752, 50 mg, store at -20 °C): FW = 12500)
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 4 mM Cytochrome c solution (dissolved in H2O):
- 1) weigh 50 mg of Cytochrome c into a small glass beaker. Difficult to weigh, since the powder is electromagnetic.
- 2) add 1 ml H2O. Dissolves easily.
- 3) divide into 0.2 ml portions
- 4) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 10 µM