Difference between revisions of "Cytochrome c"
From Bioblast
| Line 9: | Line 9: | ||
|type=Substrate ETS | |type=Substrate ETS | ||
}} | }} | ||
== Application in [[HRR]] == | |||
'''G: Glutamate''' (L-Glutamic acid, sodium salt, C<sub>5</sub>H<sub>8</sub>NO<sub>4</sub>Na; contains 1 mol/mol H<sub>2</sub>O); Sigma G 1626 (R.T.): FW = 187.1 or 169.1 anhydrous) | |||
'''Preparation of 2 M L-Glutamic acid solution''' (dissolved in H<sub>2</sub>O): | |||
::1) weigh 1.871 g of L-Glutamic acid and transfer to 5 mL volumetric glass flask | |||
::2) add 3 ml H<sub>2</sub>O (pH = 6) | |||
::3) neutralize with 5 N KOH (approx. 50 µl) | |||
::4) check pH and adjust to 7 if necessary | |||
::5) adjust final volume to 5 ml and divide into 0.5 ml portions. | |||
::6) store frozen at -20 °C | |||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | |||
::* Titration volume: 10 µl using a 25 µl syringe | |||
::* Final conc. in 2 ml O2k-chamber: 10 mM | |||
Revision as of 11:19, 22 November 2010
- high-resolution terminology - matching measurements at high-resolution
Cytochrome c
Description
Abbreviation: c
Reference: MiPNet09.12; Gnaiger_2002_BiochemSocTrans
Labels:
Application in HRR
G: Glutamate (L-Glutamic acid, sodium salt, C5H8NO4Na; contains 1 mol/mol H2O); Sigma G 1626 (R.T.): FW = 187.1 or 169.1 anhydrous)
Preparation of 2 M L-Glutamic acid solution (dissolved in H2O):
- 1) weigh 1.871 g of L-Glutamic acid and transfer to 5 mL volumetric glass flask
- 2) add 3 ml H2O (pH = 6)
- 3) neutralize with 5 N KOH (approx. 50 µl)
- 4) check pH and adjust to 7 if necessary
- 5) adjust final volume to 5 ml and divide into 0.5 ml portions.
- 6) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 10 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 10 mM
