Difference between revisions of "Cytochrome c"
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|type=Substrate ETS | |type=Substrate ETS | ||
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Revision as of 16:48, 9 March 2011
Description
Cytochrome c is a component of the electron transport system (ETS) in mitochondria. It is a small heme protein loosely associated with the outside side of the inner mitochondrial membrane. The heme group of cytochrome c transfers electrons from complex III to the complex IV. The release of cytochrome c into the cytoplasm is associated with apoptosis. [1].
Abbreviation: c
Reference: MiPNet09.12; Gnaiger_2002_BiochemSocTrans
MitoPedia topics:
Substrate and metabolite
Application in HRR
c: Cytochrome c (Sigma C 7752, 50 mg, store at -20 ยฐC): FW = 12500)
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 4 mM Cytochrome c solution (dissolved in H2O):
- 1) weigh 50 mg of Cytochrome c into a small glass beaker. Difficult to weigh, since the powder is electromagnetic.
- 2) add 1 ml H2O. Dissolves easily.
- 3) divide into 0.2 ml portions
- 4) store frozen at -20 ยฐC
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 ยตl using a 25 ยตl syringe
- Final conc. in 2 ml O2k-chamber: 10 ยตM