Difference between revisions of "Crisan 2008 Stem Cells"
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{{Publication | {{Publication | ||
|title=Crisan M, Casteilla L, Lehr L, Carmona M, Paoloni-Giacobino A, Yap S, Sun B, Leger B, Logar A, Penicaud L, Schrauwen P, Cameron-Smith D, Russell AP, Peault B, Giacobino JP (2008) A reservoir of brown adipocyte progenitors in human skeletal muscle. Stem Cells 26: 2425- | |title=Crisan M, Casteilla L, Lehr L, Carmona M, Paoloni-Giacobino A, Yap S, Sun B, Leger B, Logar A, Penicaud L, Schrauwen P, Cameron-Smith D, Russell AP, Peault B, Giacobino JP (2008) A reservoir of brown adipocyte progenitors in human skeletal muscle. Stem Cells 26:2425-33. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/18617684 PMID: 18617684 ] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/18617684 PMID: 18617684 ] | ||
|authors=Crisan M, Casteilla L, Lehr L, Carmona MC, Paoloni-Giacobino A, Yap S, Sun B, Leger B, Logar A, Penicaud L, Schrauwen P, Cameron-Smith D, Russell AP, Peault B, Giacobino JP | |authors=Crisan M, Casteilla L, Lehr L, Carmona MC, Paoloni-Giacobino A, Yap S, Sun B, Leger B, Logar A, Penicaud L, Schrauwen P, Cameron-Smith D, Russell AP, Peault B, Giacobino JP | ||
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|area=Respiration, Genetic knockout;overexpression, mt-Medicine | |area=Respiration, Genetic knockout;overexpression, mt-Medicine | ||
|organism=Human, Mouse | |organism=Human, Mouse | ||
|tissues=Endothelial; | |tissues=Endothelial;epithelial;mesothelial cell | ||
|preparations=Intact cells | |preparations=Intact cells | ||
|enzymes=Uncoupling protein | |enzymes=Uncoupling protein |
Revision as of 14:48, 17 February 2015
Crisan M, Casteilla L, Lehr L, Carmona M, Paoloni-Giacobino A, Yap S, Sun B, Leger B, Logar A, Penicaud L, Schrauwen P, Cameron-Smith D, Russell AP, Peault B, Giacobino JP (2008) A reservoir of brown adipocyte progenitors in human skeletal muscle. Stem Cells 26:2425-33. |
Crisan M, Casteilla L, Lehr L, Carmona MC, Paoloni-Giacobino A, Yap S, Sun B, Leger B, Logar A, Penicaud L, Schrauwen P, Cameron-Smith D, Russell AP, Peault B, Giacobino JP (2008) Stem Cells
Abstract: Brown adipose tissue uncoupling protein-1 (UCP1) plays a major role in the control of energy balance in rodents. It has long been thought, however, that there is no physiologically relevant UCP1 expression in adult humans. In this study we show, using an original approach consisting of sorting cells from various tissues and differentiating them in an adipogenic medium, that a stationary population of skeletal muscle cells expressing the CD34 surface protein can differentiate in vitro into genuine brown adipocytes with a high level of UCP1 expression and uncoupled respiration. These cells can be expanded in culture, and their UCP1 mRNA expression is strongly increased by cell-permeating cAMP derivatives and a peroxisome-proliferator-activated receptor-gamma (PPARgamma) agonist. Furthermore, UCP1 mRNA was detected in the skeletal muscle of adult humans, and its expression was increased in vivo by PPARgamma agonist treatment. All the studies concerning UCP1 expression in adult humans have until now been focused on the white adipose tissue. Here we show for the first time the existence in human skeletal muscle and the prospective isolation of progenitor cells with a high potential for UCP1 expression. The discovery of this reservoir generates a new hope of treating obesity by acting on energy dissipation. β’ Keywords: Brown adipocytes, Human muscle
β’ O2k-Network Lab: FR_Toulouse_Casteilla L, NL_Maastricht_Schrauwen P, ES Barcelona Gomis R
Labels: MiParea: Respiration, Genetic knockout;overexpression, mt-Medicine
Organism: Human, Mouse
Tissue;cell: Endothelial;epithelial;mesothelial cell
Preparation: Intact cells
Enzyme: Uncoupling protein
Regulation: Uncoupler
Coupling state: OXPHOS
HRR: Oxygraph-2k