Difference between revisions of "Covi 2007 Physiol Biochem Zool"
(Created page with "{{Publication |title=Covi JA, Hand SC (2007) Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase. Physio...") ย |
Beno Marija (talk | contribs) ย |
||
(12 intermediate revisions by 5 users not shown) | |||
Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Covi JA, Hand SC (2007) Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase. Physiol Biochem Zool 80: 422- | |title=Covi JA, Hand SC (2007) Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase. Physiol Biochem Zool 80:422-32. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/17508337 PMID: 17508337] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/17508337 PMID: 17508337] | ||
|authors=Covi JA, Hand SC | |authors=Covi JA, Hand SC | ||
|year=2007 | |year=2007 | ||
|journal=Physiol Biochem Zool | |journal=Physiol Biochem Zool | ||
|abstract=We examine herein the contribution of V-ATPase activity to the energy budget of aerobically developing embryos of Artemia franciscana and discuss the results in the context of quiescence under anoxia. (31)P-NMR analysis indicates that intracellular pH and NTP levels are unaffected by acute incubation of dechorionated embryos with the V-ATPase inhibitor, bafilomycin A(1). Bafilomycin A(1) also has no significant effect on oxygen consumption by isolated mitochondria. Taken together, these data indicate that bafilomycin does not affect energy-producing pathways in the developing embryo. However, the V-ATPase inhibitor exhibits a concentration-dependent inhibition of oxygen consumption in aerobic embryos. A conservative analysis of respirometric data indicates that proton pumping by the V-ATPase, and processes immediately dependent on this activity, constitutes approximately 31% of the aerobic energy budget of the preemergent embryo. Given the complete absence of detectable Na(+)K(+)-ATPase activity during the first hours of aerobic development, it is plausible that the V-ATPase is performing a role in both the acidification of intracellular compartments and the energization of plasma membranes. Importantly, the high metabolic cost associated with maintaining these diverse proton gradients requires that V-ATPase activity be downregulated under anoxia in order to attain the almost complete metabolic depression observed in the quiescent embryo. | |abstract=We examine herein the contribution of V-ATPase activity to the energy budget of aerobically developing embryos of ''Artemia franciscana'' and discuss the results in the context of quiescence under anoxia. (31)P-NMR analysis indicates that intracellular pH and NTP levels are unaffected by acute incubation of dechorionated embryos with the V-ATPase inhibitor, bafilomycin A(1). Bafilomycin A(1) also has no significant effect on oxygen consumption by isolated mitochondria. Taken together, these data indicate that bafilomycin does not affect energy-producing pathways in the developing embryo. However, the V-ATPase inhibitor exhibits a concentration-dependent inhibition of oxygen consumption in aerobic embryos. A conservative analysis of respirometric data indicates that proton pumping by the V-ATPase, and processes immediately dependent on this activity, constitutes approximately 31% of the aerobic energy budget of the preemergent embryo. Given the complete absence of detectable Na(+)K(+)-ATPase activity during the first hours of aerobic development, it is plausible that the V-ATPase is performing a role in both the acidification of intracellular compartments and the energization of plasma membranes. Importantly, the high metabolic cost associated with maintaining these diverse proton gradients requires that V-ATPase activity be downregulated under anoxia in order to attain the almost complete metabolic depression observed in the quiescent embryo. | ||
|keywords=V-ATPase, bafilomycin | |keywords=V-ATPase, bafilomycin, brine shrimp, anoxia, embryos | ||
|mipnetlab=US LA Baton Rouge Hand SC | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Comparative MiP;environmental MiP, Developmental biology | |||
|injuries=Ischemia-reperfusion | |||
|organism=Artemia, Crustaceans | |||
|preparations=Isolated mitochondria | |||
|topics=pH | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} |
Latest revision as of 09:35, 9 November 2016
Covi JA, Hand SC (2007) Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase. Physiol Biochem Zool 80:422-32. |
Covi JA, Hand SC (2007) Physiol Biochem Zool
Abstract: We examine herein the contribution of V-ATPase activity to the energy budget of aerobically developing embryos of Artemia franciscana and discuss the results in the context of quiescence under anoxia. (31)P-NMR analysis indicates that intracellular pH and NTP levels are unaffected by acute incubation of dechorionated embryos with the V-ATPase inhibitor, bafilomycin A(1). Bafilomycin A(1) also has no significant effect on oxygen consumption by isolated mitochondria. Taken together, these data indicate that bafilomycin does not affect energy-producing pathways in the developing embryo. However, the V-ATPase inhibitor exhibits a concentration-dependent inhibition of oxygen consumption in aerobic embryos. A conservative analysis of respirometric data indicates that proton pumping by the V-ATPase, and processes immediately dependent on this activity, constitutes approximately 31% of the aerobic energy budget of the preemergent embryo. Given the complete absence of detectable Na(+)K(+)-ATPase activity during the first hours of aerobic development, it is plausible that the V-ATPase is performing a role in both the acidification of intracellular compartments and the energization of plasma membranes. Importantly, the high metabolic cost associated with maintaining these diverse proton gradients requires that V-ATPase activity be downregulated under anoxia in order to attain the almost complete metabolic depression observed in the quiescent embryo. โข Keywords: V-ATPase, bafilomycin, brine shrimp, anoxia, embryos
โข O2k-Network Lab: US LA Baton Rouge Hand SC
Labels: MiParea: Respiration, Comparative MiP;environmental MiP, Developmental biology
Stress:Ischemia-reperfusion Organism: Artemia, Crustaceans
Preparation: Isolated mitochondria
Regulation: pH
HRR: Oxygraph-2k