Cormier 2019 Sci Rep: Difference between revisions
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|pathways=N, Gp, NS, ROX | |||
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Revision as of 17:20, 20 March 2019
Cormier RPJ, Champigny CM, Simard CJ, St-Coeur PD, Pichaud N (2019) Dynamic mitochondrial responses to a high-fat diet in Drosophila melanogaster. Sci Rep 9:4531. |
Cormier RPJ, Champigny CM, Simard CJ, St-Coeur PD, Pichaud N (2019) Sci Rep
Abstract: Mitochondria can utilize different fuels according to physiological and nutritional conditions to promote cellular homeostasis. However, during nutrient overload metabolic inflexibility can occur, resulting in mitochondrial dysfunctions. High-fat diets (HFDs) are usually used to mimic this metabolic inflexibility in different animal models. However, how mitochondria respond to the duration of a HFD exposure is still under debate. In this study, we investigated the dynamic of the mitochondrial and physiological functions in Drosophila melanogaster at several time points following an exposure to a HFD. Our results showed that after two days on the HFD, mitochondrial respiration as well as ATP content of thorax muscles are increased, likely due to the utilization of carbohydrates. However, after four days on the HFD, impairment of mitochondrial respiration at the level of complex I, as well as decreased ATP content were observed. This was associated with an increased contribution of complex II and, most notably of the mitochondrial glycerol-3-phosphate dehydrogenase (mG3PDH) to mitochondrial respiration. We suggest that this increased mG3PDH capacity reflects the occurrence of metabolic inflexibility, leading to a loss of homeostasis and alteration of the cellular redox status, which results in senescence characterized by decreased climbing ability and premature death.
โข Bioblast editor: Plangger M โข O2k-Network Lab: CA Rimouski Blier PU
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Organism: Drosophila
Tissue;cell: Skeletal muscle, Other cell lines
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET
Pathway: N, Gp, NS, ROX
HRR: Oxygraph-2k
Labels, 2019-03