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| {{MitoPedia
| | #REDIRECT[[Complex IV]] |
| |abbr=CIV
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| |description='''CIV''': Electron flow through [[Complex IV]] (cytochrome ''c'' oxidase) is measured in intact mitochondria after inhibiton of CIII by antimycin A, and addition of [[ascorbate]] (As) and the artificial substrate [[TMPD]] (Tm). Ascorbate has to be titrated first. It reduces [[TMPD]], which further reduces cytochrome ''c'', which is the substrate of CIV. Since CIV is a proton pump of the electron transfer system, the single step of CIV-linked respiration can be measured in different coupling states: ''L'', ''P'', and ''E''. Measurement of CIV activity requires uncoupler titrations to eliminate any potential control by the phosphorylation system, and a cytochrome ''c'' test to avoid any limitation by cytochrome ''c'' release. Total oxygen uptake in the ascorbate&TMPD(&c) stimulated state (Tm) has to be corrected for chemical background oxygen consumption.
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| |info=[[Gnaiger 2002 Biochem Soc Trans]]
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| {{MitoPedia concepts
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| |mitopedia concept=Respiratory state, SUIT state
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| == CIV(L) ==
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| == CIV(P) ==
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| == CIV(E) ==
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