Catalao 2019 Brain Res
Catalรฃo CHR, Souza AO, Santos-Jรบnior NN, da Silva SC, da Costa LHA, Alberici LC, Rocha MJA, da Silva Lopes L (2019) Kaolin-induced hydrocephalus causes acetylcholinesterase activity dysfunction following hypothalamic damage in infant rats. Brain Res 1724:146408. |
Catalao CHR, Souza AO, Santos-Jรบnior NN, da Silva SC, da Costa LHA, Alberici LC, Rocha MJA, da Silva Lopes L (2019) Brain Res
Abstract: In hydrocephalus, the progressive accumulation of cerebrospinal fluid (CSF) causes dilatation of the lateral ventricles affecting the third ventricle and diencephalic structures such as the hypothalamus. These structures play a key role in the regulation of several neurovegetative functions by the production of the hormones. Since endocrine disturbances are commonly observed in hydrocephalic children, we investigated the impact of progressive ventricular dilation on the hypothalamus of infant rats submitted to kaolin-induced hydrocephalus. Seven-day-old infant rats were submitted to hydrocephalus induction by kaolin 20% injection method. After 14โฏdays, the animals were decapitated and brain was collected to analyze mitochondrial function, neuronal activity by acetylcholinesterase (AChE) enzyme, oxidative damage, glial activation, and, neurotransmission-related proteins and anti-apoptotic processes in the hypothalamus. The hydrocephalic animals showed reduction in respiratory rates in the States of phosphorylation (Pโฏ<โฏ0.01) and non-phosphorylation (Pโฏ<โฏ0.05); increase in AChE activity in both the cytosol (Pโฏ<โฏ0.05) and the membrane (Pโฏ<โฏ0.01); decrease in synaptophysin (Pโฏ<โฏ0.05) and Bcl-2 (Pโฏ<โฏ0.05) contents and; increase in protein carbonyl (Pโฏ<โฏ0.01), GFAP (Pโฏ<โฏ0.01) and Iba-1 (Pโฏ<โฏ0.05) levels. The results demonstrate that ventricular dilation causes hypothalamic damage characterized by cholinergic dysfunction and suggests further investigation of the synthesis and secretion of hormones to generate new approaches and to assist in the treatment of hydrocephalic patients with hormonal alterations.
Copyright ยฉ 2019 Elsevier B.V. All rights reserved. โข Keywords: Acetylcholinesterase enzyme, Experimental hydrocephalus, Hypothalamus, Neuroendocrine dysfunction โข Bioblast editor: Plangger M
Labels: MiParea: Respiration
HRR: Oxygraph-2k
2020-04