Carnitine: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
::::* Car:ย '''L-carnitine:''' Sigma Aldrich; C0158; | ::::* Car:ย '''L-carnitine:''' Sigma Aldrich; C0158; M = 161.2 gยทmol<sup>-1</sup> | ||
::::'''Preparation of 250 mM stock solution (dissolved in H<sub>2</sub>O):''' | ::::'''Preparation of 250 mM stock solution (dissolved in H<sub>2</sub>O):''' | ||
::::# Weigh 100 mg of Carnitine. | ::::# Weigh 100 mg of Carnitine. | ||
Revision as of 13:14, 12 February 2021
- high-resolution terminology - matching measurements at high-resolution
Carnitine
Description
Carnitine is an important factor for the transport of long-chain fatty acids bound to carnitine (carnitine acyltransferase) into the mitochondrial matrix for subsequent ฮฒ-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.
Abbreviation: Car
Application in HRR
- Car: L-carnitine: Sigma Aldrich; C0158; M = 161.2 gยทmol-1
- Preparation of 250 mM stock solution (dissolved in H2O):
- Weigh 100 mg of Carnitine.
- Add 2.48 mL H2O.
- Divide into 100 ยตL portions.
- Store frozen at -20ยฐC.
- O2k Manual titration
- Titration volume: 4 ยตL 250 mM stock using a 10 ยตL Hamilton syringe (2 mL O2k-Chamber).
- Final concentration: 0.5 mM.
- O2k Manual titration
Feeding carnitine
MitoPedia topics:
Substrate and metabolite
