Difference between revisions of "Burtscher 2015 Mitochondrion"
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|title=Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E (2015) Differences in mitochondrial function in homogenated samples from healthy and epileptic specific brain tissues revealed by high-resolution respirometry. Mitochondrion 25:104-12. | |title=Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E (2015) Differences in mitochondrial function in homogenated samples from healthy and epileptic specific brain tissues revealed by high-resolution respirometry. Mitochondrion 25:104-12. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/26516105 PMID: 26516105 Open Access] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/26516105 PMID: 26516105 Open Access] | ||
|authors=Burtscher J, Zangrandi L, Schwarzer C, Gnaiger | |authors=Burtscher J, Zangrandi L, Schwarzer C, Gnaiger Erich | ||
|year=2015 | |year=2015 | ||
|journal=Mitochondrion | |journal=Mitochondrion | ||
| Line 12: | Line 12: | ||
In summary, respirometric OXPHOS analysis provides a very sensitive diagnostic approach using small amounts of distinct brain tissues. In a single assay, information is obtained on numerous OXPHOS parameters as indicators of tissue-specific mitochondrial performance. | In summary, respirometric OXPHOS analysis provides a very sensitive diagnostic approach using small amounts of distinct brain tissues. In a single assay, information is obtained on numerous OXPHOS parameters as indicators of tissue-specific mitochondrial performance. | ||
<br><br> | |||
|keywords=Neurodegeneration, Epilepsy, Mitochondria, Brain regions, Respirometry | |keywords=Neurodegeneration, Epilepsy, Mitochondria, Brain regions, Respirometry | ||
|editor=[[Gnaiger E]] | |editor=[[Gnaiger E]] | ||
|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck | |mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Oroboros | ||
}} | }} | ||
[[Image:Logo MitoFit.jpg|right|120px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]] | |||
== MitoFit news 2015#17 == | |||
::::* 2015-11-16: MitoFit in the brain. » [[K-Regio_MitoFit#2015 |MitoFit news]] | |||
== Cited by == | |||
::* 23 articles in PubMed (2021-12-27) https://pubmed.ncbi.nlm.nih.gov/26516105/ | |||
{{Labeling | {{Labeling | ||
|area=Respiration, mt-Biogenesis;mt-density, Pharmacology;toxicology | |area=Respiration, mt-Biogenesis;mt-density, Pharmacology;toxicology | ||
|diseases=Neurodegenerative | |||
|organism=Mouse | |organism=Mouse | ||
|tissues=Nervous system | |tissues=Nervous system | ||
|preparations=Permeabilized tissue, Homogenate | |preparations=Permeabilized tissue, Homogenate | ||
|couplingstates=LEAK, OXPHOS, ET | |||
|couplingstates=LEAK, OXPHOS, | |||
|pathways=N, S, CIV, NS, ROX | |pathways=N, S, CIV, NS, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=PBI-Shredder | |additional=PBI-Shredder | ||
}} | }} | ||
Latest revision as of 12:58, 27 December 2021
| Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E (2015) Differences in mitochondrial function in homogenated samples from healthy and epileptic specific brain tissues revealed by high-resolution respirometry. Mitochondrion 25:104-12. |
Burtscher J, Zangrandi L, Schwarzer C, Gnaiger Erich (2015) Mitochondrion
Abstract: Mitochondrial dysfunction and oxidative stress are strongly implicated in neurodegenerative diseases and epilepsy. Strikingly, neurodegenerative diseases show regional specificity in vulnerability and follow distinct patterns of neuronal loss. A challenge is to understand, why mitochondria fail in particular brain regions under specific pathological conditions. A potential explanation could be provided by regional or cellular specificity of mitochondrial function.
We applied high-resolution respirometry to analyze the integrated Complex I- and II (CI and CII)-linked respiration, the activity of Complex IV, and the combined CI&II-linked oxidative phosphorylation (OXPHOS)- and electron-transfer system (ET-pathway)-capacity in microsamples obtained from distinct regions of the mouse brain. We compared different approaches to assess mitochondrial density and suggest flux control ratios as a valid method to normalize respiration to mitochondrial density.
This approach revealed significant differences of CI- and CII-linked OXPHOS capacity and coupling control between motor cortex, striatum, hippocampus and pons of naïve mice. CI-linked respiration was highest in motor cortex, while CII-linked respiration predominated in the striatum. To investigate if this method could also determine differences in normal and disease states within the same brain region, we compared hippocampal homogenates in a chronic epilepsy model. Three weeks after stereotaxic injection of kainate, there was a down-regulation of CI- and upregulation of CII-linked respiration in the resulting epileptic ipsilateral hippocampus compared to the contralateral one.
In summary, respirometric OXPHOS analysis provides a very sensitive diagnostic approach using small amounts of distinct brain tissues. In a single assay, information is obtained on numerous OXPHOS parameters as indicators of tissue-specific mitochondrial performance.
• Keywords: Neurodegeneration, Epilepsy, Mitochondria, Brain regions, Respirometry
• Bioblast editor: Gnaiger E
• O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck Oroboros
MitoFit news 2015#17
- 2015-11-16: MitoFit in the brain. » MitoFit news
Cited by
- 23 articles in PubMed (2021-12-27) https://pubmed.ncbi.nlm.nih.gov/26516105/
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Pharmacology;toxicology
Pathology: Neurodegenerative
Organism: Mouse Tissue;cell: Nervous system Preparation: Permeabilized tissue, Homogenate
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, CIV, NS, ROX
HRR: Oxygraph-2k
PBI-Shredder
