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Difference between revisions of "Bioblast alert 2011"

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'''Bioblast alert''' is prepared by the [[Bioblast editorial board]] to draw attention to specific topics on the '''Bioblast wiki'''.
'''[[Bioblast alert|back to "Bioblast alert" main page]]'''
'''Bioblast alert''': for information purposes, not intended to be an advertisement. 


__TOC__
=== Bioblast alert 2011(01): 2011-11-11 ===


* A key question on the separation of '''qualitative versus quantitative change of mitochondrial function''' is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?
'''Bioblast alert 2011: 01 to 04''' ->> [[Bioblast alert|Current issue]]
::[[Pesta_2011_Am J Physiol Regul Integr Comp Physiol|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.]]
=== Bioblast alert 2011(04): 2011-12-24 ===
* [[ET capacity]] plays a key role in such analyses, which cannot be achieved quantitatively without careful '''[[Optimum uncoupler concentration|uncoupler titrations]]'''.
* '''[[Basal respiration]]''' - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology.
 
 
=== Bioblast alert 2011(02): 2011-11-17 ===


* '''Congratulations''' to [[Pesta D|Dr. Dominik Pesta]], for receiving his degree of a PhD at the Medical University of Innsbruck! (Jan 2012)
* '''[[MiPNet16.02 IOC64|IOC64 - Centre for Cellular and Molecular Biology, Hyderabad, India]]: 2011-12-08'''.
* [[Bioblast editorial board]] - [[Talk:Bioblast editorial board|Why a wiki editorial board?]]
* '''[[SUIT protocol]]s for HRR: from intact cells to permeabilized muscle fibres''':
::[[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]]


* '''Heart failure and mitochondrial dysfunction'''. Increased [[OXPHOS capacity]] after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.
::[[Lemieux 2011 Int J Biochem Cell Biol|Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738.]] --> [[Talk:Lemieux 2011 Int J Biochem Cell Biol|Discussion: Complementary results on the PGC-1a pathway]].


=== Bioblast alert 2011(03): 2011-11-24 ===
* '''[[Tissue storage]]''' on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with [[SUIT protocol]]s.
* '''Fluorometry combined with high-resolution respirometry''': Light guide inserted into the chamber through a black PEEK stopper<ref>[[Anderson 2011 Am J Physiol Heart Circ Physiol|Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300: H118-124.]]</ref>, or optical probe operated through the O2k-glass window<ref>[[Hickey 2012 J Comp Physiol B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (''Hemiscyllum ocellatum''). J Comp Physiol B [Epub ahead of print] ]]</ref>.
<references/>


*'''[[Talk:Fluorometry|Fluorometry versus fluorimetry]]''' - term of the week. >>>New '''[[Glossary: Fluorometry]]''' - high-resolution terminology.
* '''[[Uncoupled]], [[noncoupled]], [[dyscoupled]]''' - term of the week: a hallmark of high-resolution respirometry is a matching high-resolution terminology.
::[[Talk:Uncoupled respiration|Evaluate an example of low-resolution terminology]]: '''''[[Dyscoupled respiration|Uncoupling]]''' was studied in stressed cells by measurement of coupled respiration, evaluation of '''[[LEAK respiration|uncoupled]]''' respiration after inhibition with oligomycin, and FCCP titration to quantify '''[[noncoupled|uncoupled]]''' respiration''.




=== Bioblast alert 2011(02): 2011-11-17 ===


* '''[http://www.oroboros.at/index.php?ioc64 O2k-Workshop IOC64 - Centre for Cellular and Molecular Biology, Hyderabad, India]: 2011-12-08'''.
=== Bioblast alert 2011(03): 2011-11-24 ===
* '''Fluorometry combined with high-resolution respirometry''': Light guide inserted into the chamber through a black PEEK stopper<ref>[[Anderson 2011 Am J Physiol Heart Circ Physiol|Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300:H118-24.]]</ref>, or optical probe operated through the O2k-glass window<ref>[[Hickey 2012 J Comp Physiol B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (''Hemiscyllum ocellatum''). J Comp Physiol B 182:91-100. ]]</ref>.
<references/>


* '''Heart failure and mitochondrial dysfunction'''. Increased [[OXPHOS capacity]] after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.  
*'''[[Talk:Fluorometry|Fluorometry versus fluorimetry]]''' - term of the week. >>>New '''[[:MitoPedia: Fluorometry]]''' - high-resolution terminology.
::[[Lemieux 2011 Int J Biochem Cell Biol|Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738.]] --> [[Talk:Lemieux 2011 Int J Biochem Cell Biol|Discussion: Complementary results on the PGC-1a pathway]].


* '''[[Tissue storage]]''' on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with [[SUIT protocol]]s. ([[Scientific service]])
=== Bioblast alert 2011(04): 2011-12-24 ===


* '''[[Uncoupled]], [[non-coupled]], [[dyscoupled]]''' - term of the week: a hallmark of high-resolution respirometry is a matching high-resolution terminology.
* '''Congratulations''' to [[Pesta D|Dr. Dominik Pesta]], for receiving his degree of a PhD at the Medical University of Innsbruck! (Jan 2012)
::[[Talk:Uncoupled respiration|Evaluate an example of low-resolution terminology]]: '''''[[Dyscoupled respiration|Uncoupling]]''' was studied in stressed cells by measurement of coupled respiration, evaluation of '''[[LEAK|uncoupled]]''' respiration after inhibition with oligomycin, and FCCP titration to quantify '''[[non-coupled|uncoupled]]''' respiration''.
* [[Bioblast editorial board]] - [[Talk:Bioblast editorial board|Why a wiki editorial board?]]  
* '''[[SUIT protocol]]s for HRR: from intact cells to permeabilized muscle fibres''':
::[[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58.]]




=== Bioblast alert 2011(01): 2011-11-11 ===


* A key question on the separation of '''qualitative versus quantitative change of mitochondrial function''' is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?
::[[Pesta_2011_Am J Physiol Regul Integr Comp Physiol|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.]]
* [[ETS capacity]] plays a key role in such analyses, which cannot be achieved quantitatively without careful '''[[Optimum uncoupler concentration|uncoupler titrations]]'''.
* '''[[Basal respiration]]''' - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology.
[[Category:Gentle Science]]
[[Category:Gentle Science]]

Latest revision as of 12:24, 25 November 2020

Bioblast alert 2011

Bioblast wiki
Bioblast alert

back to "Bioblast alert" main page

Bioblast alert 2011(01): 2011-11-11

  • A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?
Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.
  • ET capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations.
  • Basal respiration - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology.


Bioblast alert 2011(02): 2011-11-17

  • Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.
Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738. --> Discussion: Complementary results on the PGC-1a pathway.
  • Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols.
Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.


Bioblast alert 2011(03): 2011-11-24

  • Fluorometry combined with high-resolution respirometry: Light guide inserted into the chamber through a black PEEK stopper[1], or optical probe operated through the O2k-glass window[2].
  1. ↑ Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300:H118-24.
  2. ↑ Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182:91-100.

Bioblast alert 2011(04): 2011-12-24

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58.
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