Description
Antimycin A is an inhibitor of Complex III (CIII). It binds to the Qi site of CIII and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor). Antimycin A also inhibits acyl-CoA oxidase.
Abbreviation: Ama
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRFR
- Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540
- Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):
- Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
- Add 2 ml ethanol.
- Divide into 0.2 ml portions in glass vials.
- Store at -20 °C.
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 2.5 µM.
Compare
- Myxothiazol - this CIII inhibitor induces less ROS production but is more expensive than Ama.
Antimycin A and ROS production
- Is hydrogen peroxide production measured after inhibition of the Electron transfer-pathway by rotenone and antimycin A of diagnostic significance? - Please, share your evidence (Email to [email protected]).
Inhibition of acyl-CoA oxidase
- Vamecq J, Schepers L, Parmentier G, Mannaerts GP (1987) Inhibition of peroxisomal fatty acyl-CoA oxidase by antimycin A. Biochem J 248:603-7. - »Bioblast link«