Difference between revisions of "Antimycin A"
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Revision as of 16:49, 9 March 2011
- high-resolution terminology - matching measurements at high-resolution
Antimycin A
Description
Inhibitor of Complex III. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor)[1].
Abbreviation: Ama
Reference: MiPNet09.12,
MitoPedia topics:
Inhibitor
Application in HRR
Ama: Antimycin A (Antimycin A from Streptomyces sp., Sigma A 8674, 25 g, store at -20 °C: FW = 540)
Caution: Very toxic!!! Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 5 mM stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 5.4 mg of Antimycin A into a small glass vial !Difficult to weigh - appears to be hygroscopic!
- 2) add 2 ml Ethanol
- 3) divide into 0.2 ml portions (use glass vials)
- 4) store at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 2.5 µM
