Difference between revisions of "Antimycin A"
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== Application in [[ | == Application in [[HRR]] == | ||
::: '''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 mg, store at -20 °C: FW = 540 | ::: '''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 mg, store at -20 °C: FW = 540 | ||
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::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber). | ::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber). | ||
::::* Final concentration: 2.5 µM. | ::::* Final concentration: 2.5 µM. | ||
:::'''Microalgae applications''' | |||
::: For the specific case of microalgal cells it is recommended to prepare higher concentration: | |||
::: '''Preparation of 10 mM stock solution''' (dissolved in 99.9% ethanol) | |||
::::# Weigh 10.8 mg of antimycin A and dissolve in 2 ml of ethanol absolute (99.9%). | |||
::::# Divide into 0.2 mL portions in clear glass vials. | |||
::::# Store at -20 °C. | |||
::: '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume: 8 µL using a 10 µL syringe (2 mL O2k-Chamber). | |||
::::* Final concentration: 40 µM. | |||
Revision as of 12:17, 15 April 2019
Description
Antimycin A is an inhibitor of Complex III (CIII). It binds to the Qi site of CIII and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor). High concentrations of antimycin A also inhibit acyl-CoA oxidase and D-amino acid oxidase.
Abbreviation: Ama
Reference: MiPNet09.12
MitoPedia topics:
Inhibitor
Application in HRR
- Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540
- Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):
- Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
- Add 2 mL ethanol.
- Divide into 0.2 mL portions in clear glass vials.
- Store at -20 °C.
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber).
- Final concentration: 2.5 µM.
- Microalgae applications
- For the specific case of microalgal cells it is recommended to prepare higher concentration:
- Preparation of 10 mM stock solution (dissolved in 99.9% ethanol)
- Weigh 10.8 mg of antimycin A and dissolve in 2 ml of ethanol absolute (99.9%).
- Divide into 0.2 mL portions in clear glass vials.
- Store at -20 °C.
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 8 µL using a 10 µL syringe (2 mL O2k-Chamber).
- Final concentration: 40 µM.
Compare
- Myxothiazol - this CIII inhibitor induces less ROS production but is more expensive than Ama.
Antimycin A and ROS production
- Is hydrogen peroxide production measured after inhibition of the Electron transfer-pathway by rotenone and antimycin A of diagnostic significance? - Please, share your evidence (Email to [email protected]).
Inhibition of acyl-CoA oxidase and D-amino acid oxidase
- Vamecq J, Schepers L, Parmentier G, Mannaerts GP (1987) Inhibition of peroxisomal fatty acyl-CoA oxidase by antimycin A. Biochem J 248:603-7. - »Bioblast link«