Antimycin A

high-resolution terminology - matching measurements at high-resolution

Antimycin A

Description

Inhibitor of Complex III. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor)[1].

Abbreviation: Ama

Reference: MiPNet09.12,

Labels:

Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Flux Control; Additivity; Threshold; Excess Capacity"Flux Control; Additivity; Threshold; Excess Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 

HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

Application in HRR

Ama: Antimycin A (Antimycin A from Streptomyces sp., Sigma A 8674, 25 g, store at -20 °C: FW = 540)

Caution: Very toxic!!! Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 5 mM stock solution (dissolved in 99.9% Ethanol):

1) weigh 5.4 mg of Antimycin A into a small glass vial !Difficult to weigh - appears to be hygroscopic!

2) add 2 ml Ethanol

3) divide into 0.2 ml portions (use glass vials)

4) store at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

• Titration volume: 1 µl using a 10 µl syringe
• Final conc. in 2 ml O2k-chamber: 2.5 µM