Difference between revisions of "Antimycin A"
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{{MitoPedia | {{MitoPedia | ||
|abbr=Ama | |abbr=Ama | ||
|description=Inhibitor of [[Complex III]]. | |description=Inhibitor of [[Complex III]]. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor)[http://en.wikipedia.org/wiki/Coenzyme_Q_-_cytochrome_c_reductase#Inhibitors_of_complex_III]. | ||
|info=[[MiPNet09.12]], | |info=[[MiPNet09.12]], | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Ama: Antimycin A''' (Antimycin A from ''Streptomyces'' sp., Sigma A 8674, 25 g, store at -20 °C | '''Ama: Antimycin A''' (Antimycin A from ''Streptomyces'' sp., Sigma A 8674, 25 g, store at -20 °C: FW = 540) | ||
<span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ||
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'''Preparation of 5 mM stock solution''' (dissolved in 99.9% Ethanol): | '''Preparation of 5 mM stock solution''' (dissolved in 99.9% Ethanol): | ||
::1) weigh 5.5 mg of Antimycin A directly into a | ::1) weigh 5.5 mg of Antimycin A directly into a small glass vial !Difficult to weigh - appears to be hygroscopic! | ||
::2) add 2 ml Ethanol | ::2) add 2 ml Ethanol | ||
::3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution | ::3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution | ||
Revision as of 13:22, 3 December 2010
- high-resolution terminology - matching measurements at high-resolution
Antimycin A
Description
Inhibitor of Complex III. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor)[1].
Abbreviation: Ama
Reference: MiPNet09.12,
Labels:
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Flux Control; Additivity; Threshold; Excess Capacity"Flux Control; Additivity; Threshold; Excess Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
Ama: Antimycin A (Antimycin A from Streptomyces sp., Sigma A 8674, 25 g, store at -20 °C: FW = 540)
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 5 mM stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 5.5 mg of Antimycin A directly into a small glass vial !Difficult to weigh - appears to be hygroscopic!
- 2) add 2 ml Ethanol
- 3) divide into 0.2 ml portions OR store in 2 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 4) store in glass vials (recommendation) at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 2.5 µM
