Talk:O2k-Fluo LED2-Module

Oxygraph Series A to C

Question: Why can only one chamber be used for fluoresecence measurements with O2k Series A to C?

Answer: The oxygen channel from one chamber will be used to record the fluorescence signal form the other channel. Therefore only signals from one chamber can be recorded.

Question: What are the performance limitations when using the O2k-Fluorescence Module with O2k Serial A to C?

Answer:

Only one chamber can be used for simultaneous fluorescence and respiration measurements.
The amplification of the fluorescence signal is done outside the oxygraph in a special amplification box. The resulting signal stability is usually good and the same approach has been used to measure NO for O2k series A to C. However, signal stability may not reach the high level achieved when using O2k Series D and above.
Performing the measurement and analyzing the data is a little bit different from the way it can be done with O2k Series D and above. The fluorescence Signal will actually be found in the signal labeled "oxygen" from the unused chamber and different amplification levels will be available. The situation is very similar to measuring NO with O2k Series A to C, see H MiPNet15.05 NO-Manual.

Fasching Mario 11:55, 25 January 2012 (CET)

Simultaneous measurement of TPP and fluorescence

Question: Will it be possible to measure JO2, fluorescence AND TPP simultaneously, or just JO2/fluorescence vs JO2/TPP?

Answer: We do not know yet. The main unknown is the chemical compatibility of the methods. We still have to find out whats the effect of Ampler red and its reaction product on the TPP signal. If this is not a big problem there are technical issues: a black TPP stopper will be required and then still light entering the chamber through the TPP electrode might be a problem. Producing the TPP electrodes in black is presumable not a very good idea because then it will no longer be possible to "see" the position of the membrane. I think the technical problems are solvable but first we have to check for chemical compatibility. best greetings Mario

--Fasching Mario 10:27, 14 February 2012 (CET)