MiPNet08.18 LactateDehydrogenase: Difference between revisions
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{{Publication | {{Publication | ||
|title=Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr Physiol Network 08.18. | |title=Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr Physiol Network 08.18. | ||
|info='''Open Access - [ | |info=[[Media:MiPNet08.18 LDH.pdf|'''Open Access-MiPNet08.18]]; [http://www.bioblast.at/index.php/File:MiPNet08.18_LDH.pdf Versions] [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]] | ||
|year= | |year=2003 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=Lactate dehydrogenase (EC 1.1.1.27) is an enzyme, which catalyzes the last step in glycolysis. LDH is a soluble enzyme and localized in the cytosol (cytoplasm). LDH, therefore, is used as a quantitative marker enzyme for the intact cell, its activity providing information on cellular glycolytic capacity (Renner et al, 2003). Measurement of LDH release (leakage) is an important and frequently applied test for cellular membrane permeabilization (rupture) and severe irreversible cell damage. LDH leakage normally correlates well with CK release and the trypan blue viability test. | |abstract='''O2k-Protocol for Lactate Dehydrogenase Cytosolic Marker Enzyme ''' | ||
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Lactate dehydrogenase (EC 1.1.1.27) is an enzyme, which catalyzes the last step in glycolysis. LDH is a soluble enzyme and localized in the cytosol (cytoplasm). LDH, therefore, is used as a quantitative marker enzyme for the intact cell, its activity providing information on cellular glycolytic capacity (Renner et al, 2003). Measurement of LDH release (leakage) is an important and frequently applied test for cellular membrane permeabilization (rupture) and severe irreversible cell damage. LDH leakage normally correlates well with CK release and the trypan blue viability test. | |||
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:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | |||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | |||
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|keywords=Enzymes | |keywords=Enzymes | ||
|mipnetlab=AT_Innsbruck_OROBOROS | |mipnetlab=AT_Innsbruck_OROBOROS | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Instruments;methods | |area=Instruments;methods | ||
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|articletype=Protocol; Manual, MiPNet-online Publication | |articletype=Protocol; Manual, MiPNet-online Publication | ||
}} | }} | ||
== Further information == | |||
:>> [http://www.oroboros.at/?protocosl_ldh MiPNet08.18] |
Revision as of 15:25, 17 October 2013
Kuznetsov AV, Gnaiger E. Laboratory protocol: Lactate dehydrogenase. Cytosolic marker enzyme. Mitochondr Physiol Network 08.18. |
ยป Open Access-MiPNet08.18; Versions
Abstract: O2k-Protocol for Lactate Dehydrogenase Cytosolic Marker Enzyme
Lactate dehydrogenase (EC 1.1.1.27) is an enzyme, which catalyzes the last step in glycolysis. LDH is a soluble enzyme and localized in the cytosol (cytoplasm). LDH, therefore, is used as a quantitative marker enzyme for the intact cell, its activity providing information on cellular glycolytic capacity (Renner et al, 2003). Measurement of LDH release (leakage) is an important and frequently applied test for cellular membrane permeabilization (rupture) and severe irreversible cell damage. LDH leakage normally correlates well with CK release and the trypan blue viability test.
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
โข Keywords: Enzymes
โข O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
Preparation: Enzyme
Enzyme: Marker Enzyme"Marker Enzyme" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Enzymes
Further information
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