Vrbacky 2003 Physiol Res: Difference between revisions
Harrison DK (talk | contribs) m (moved Vrbacky 2003 PhysiolRes to Vrbacky 2003 Physiol Res) |
Harrison DK (talk | contribs) No edit summary |
||
Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Vrbacky M, Krijt J, Drahota Z, Melkova Z (2003) Inhibitory effects of Bcl-2 on mitochondrial respiration. Physiol | |title=Vrbacky M, Krijt J, Drahota Z, Melkova Z (2003) Inhibitory effects of Bcl-2 on mitochondrial respiration. Physiol Res 52: 545-554. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/14535829 PMID: 14535829] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/14535829 PMID: 14535829] | ||
|authors=Vrbacky M, Krijt J, Drahota Z, Melkova Z | |authors=Vrbacky M, Krijt J, Drahota Z, Melkova Z |
Revision as of 16:19, 13 December 2011
Vrbacky M, Krijt J, Drahota Z, Melkova Z (2003) Inhibitory effects of Bcl-2 on mitochondrial respiration. Physiol Res 52: 545-554. |
ยป [[Has info::PMID: 14535829]]
Vrbacky M, Krijt J, Drahota Z, Melkova Z (2003) Physiol. Res.
Abstract: In contrast to the well-established anti-apoptotic effect of Bcl-2 protein, we have recently demonstrated that Bcl-2 overexpression by vaccinia virus causes apoptosis in BSC-40 cells, while it prevents apoptosis in HeLa G cells. Given the key role of mitochondria in the process of apoptosis, we focused on effects of Bcl-2 expression on mitochondrial energetics of these two cell lines. In this study we present data indicating that BSC-40 cells derive their ATP mainly from oxidative phosphorylation whereas HeLa G cells from glycolysis. More importantly, we show that in both cell lines, Bcl-2 inhibits mitochondrial respiration and causes a decrease of the ATP/ADP ratio. However, it appears that BSC-40 cells cannot sustain this decrease and die, while HeLa G cells survive, being adapted to the low ratio of ATP/ADP maintained by glycolysis. Based on this observation, we propose that the outcome of Bcl-2 expression is determined by the type of cellular ATP synthesis, namely that Bcl-2 causes apoptosis in cells relying on oxidative phosphorylation. โข Keywords: Bcl-2, Apoptosis, ATP, Mitochondrial respiration
โข O2k-Network Lab: CZ Prague Houstek J
Labels:
Stress:Cancer; Apoptosis; Cytochrome c Organism: Human, Other Mammal Tissue;cell: Kidney, Endothelial; Epithelial; Mesothelial Cell Preparation: Permeabilized Cell or Tissue; Homogenate
HRR: Oxygraph-2k
monkey