Ap5A: Difference between revisions
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|abbr=Ap5A | |abbr=Ap5A | ||
|description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP โ 2 ADP). | |description='''P1,P5-Di(adenosine-5')pentaphosphate (Ap5A)''' is an inhibitor of [[adenylate kinase]] (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP โ 2 ADP). | ||
ย | }} | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |||
}} | |||
__TOC__ | __TOC__ | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
:::: '''Ap5A: P1,P5-Di(adenosine-5โฒ) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 ยฐC; molecular weight: 1026.28 | :::: '''Ap5A: P1,P5-Di(adenosine-5โฒ) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 ยฐC; molecular weight: 1026.28 | ||
::::ย <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ::::ย <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ||
ย | :::: '''Preparation of 100 mM stock solution''' (dissolved in H2O) | ||
ย | :::::# For a 2 mg glass vial: dissolve in 19.49 ยตL H2O::::# Store at -20 ยฐC. | ||
ย | |||
:::: '''Preparation of 100 mM stock solution''' (dissolved in H2O): | |||
ย | |||
::::# For a 2 mg glass vial: dissolve in 19.49 ยตL H2O | |||
::::# Store at -20 ยฐC. | |||
ย | |||
ย | |||
:::ยป '''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | :::ยป '''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume: 1 ยตL using a 10 ยตL syringe (2 mL O2k-Chamber). | ::::* Titration volume: 1 ยตL using a 10 ยตL syringe (2 mL O2k-Chamber). | ||
::::* Final concentration: 50 ยตM. | ::::* Final concentration: 50 ยตM.== References == | ||
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== References == | |||
::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980] | ::::* [https://www.ncbi.nlm.nih.gov/pubmed/6252207 Kurebayashi ''et al.'', J Biochem. 1980] | ||
::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014] | ::::* [https://www.ncbi.nlm.nih.gov/pubmed/24862274 Chinopoulos ''et al.'', Methods Enzymol. 2014] | ||
Revision as of 15:20, 30 November 2020
Description
P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP โ 2 ADP).
Abbreviation: Ap5A
MitoPedia topics: Inhibitor
Application in HRR
- Ap5A: P1,P5-Di(adenosine-5โฒ) pentaphosphate pentasodium salt Sigma D4022, store at -20 ยฐC; molecular weight: 1026.28
- Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- Preparation of 100 mM stock solution (dissolved in H2O)
- For a 2 mg glass vial: dissolve in 19.49 ยตL H2O::::# Store at -20 ยฐC.
- ยป O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 ยตL using a 10 ยตL syringe (2 mL O2k-Chamber).
- Final concentration: 50 ยตM.== References ==
- Kurebayashi et al., J Biochem. 1980
- Chinopoulos et al., Methods Enzymol. 2014