Creatine: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Cr | |abbr=Cr | ||
|description='''Creatine'''ย is a nitrogenous organic acid that occurs naturally in vertebrates and helps | |description='''Creatine'''ย is a nitrogenous organic acid that occurs naturally in vertebrates and helps primarily muscle cells to supply energy by increasing the formation of adenosine triphosphate ([[ATP]]). | ||
|info=[[Saks 2000 Acta Physiol Scand]] | |info=[[Saks 2000 Acta Physiol Scand]] | ||
}} | }} | ||
{{MitoPedia | {{MitoPedia methods}} | ||
__TOC__ | |||
== Application in [[HRR]] == | |||
= | ::: '''Cr: Creatine ''' (creatine monohydrate, C<sub>4</sub>H<sub>9</sub>N<sub>3</sub>O<sub>2</sub>*H<sub>2</sub>O ); M = 149.15 gยทmol<sup>-1</sup>; Sigma 27900, 100 g, store at RT. | ||
:::: '''Preparation of MiR05Cr or MiR06Cr with 20 mM creatine''': | |||
''' | :::: <span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span> | ||
::::# Weigh 6 mg creatine. | |||
ย | ::::# Dissolve in 2 mL [[MiR05]] or [[MiR06]] to obtain [[MiR05Cr]] or [[MiR06Cr]]. | ||
:: | {{MitoPedia topics | ||
:: | |mitopedia topic=Substrate and metabolite | ||
}} |
Latest revision as of 13:12, 12 February 2021
Description
Creatine is a nitrogenous organic acid that occurs naturally in vertebrates and helps primarily muscle cells to supply energy by increasing the formation of adenosine triphosphate (ATP).
Abbreviation: Cr
Reference: Saks 2000 Acta Physiol Scand
Application in HRR
- Cr: Creatine (creatine monohydrate, C4H9N3O2*H2O ); M = 149.15 gยทmol-1; Sigma 27900, 100 g, store at RT.
- Preparation of MiR05Cr or MiR06Cr with 20 mM creatine:
- Caution: Prepare fresh everyday!
MitoPedia topics: Substrate and metabolite