Verma 2023 Int J Mol Sci

From Bioblast
Publications in the MiPMap
Verma A, Azhar G, Zhang X, Patyal P, Kc G, Sharma S, Che Y, Wei JY (2023) P. gingivalis-LPS induces mitochondrial dysfunction mediated by neuroinflammation through oxidative stress. Int J Mol Sci 24:950.

Β» PMID: 36674463 Open Access

Verma Ambika, Azhar Gohar, Zhang Xiaomin, Patyal Pankaj, Kc Grishma, Sharma Shakshi, Che Yingni, Wei Jeanne Y (2023) Int J Mol Sci

Abstract: Porphyromonas gingivalis (P. gingivalis), a key pathogen in periodontitis, is associated with neuroinflammation. Periodontal disease increases with age; 70.1% of adults 65 years and older have periodontal problems. However, the P. gingivalis- lipopolysaccharide (LPS)induced mitochondrial dysfunction in neurodegenerative diseases remains elusive. In this study, we investigated the possible role of P. gingivalis-LPS in mitochondrial dysfunction during neurodegeneration. We found that P. gingivalis-LPS treatment activated toll-like receptor (TLR) 4 signaling and upregulated the expression of Alzheimer's disease-related dementia and neuroinflammatory markers. Furthermore, the LPS treatment significantly exacerbated the production of reactive oxygen species and reduced the mitochondrial membrane potential. Our study highlighted the pivotal role of P. gingivalis-LPS in the repression of serum response factor (SRF) and its co-factor p49/STRAP that regulate the actin cytoskeleton. The LPS treatment repressed the genes involved in mitochondrial function and biogenesis. P. gingivalis-LPS negatively altered oxidative phosphorylation and glycolysis and reduced total adenosine triphosphate (ATP) production. Additionally, it specifically altered the mitochondrial functions in complexes I, II, and IV of the mitochondrial electron transport chain. Thus, it is conceivable that P. gingivalis-LPS causes mitochondrial dysfunction through oxidative stress and inflammatory events in neurodegenerative diseases. β€’ Keywords: P. gingivalis-LPS, Mitochondrial dysfunction, Neuroinflammation, Oxidative stress β€’ Bioblast editor: Plangger M

Labels: MiParea: Respiration  Pathology: Neurodegenerative 

Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell  Preparation: Permeabilized cells, Intact cells 

Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: N, S, CIV, ROX  HRR: Oxygraph-2k 


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