Tyrrell 2016 Redox Biol

From Bioblast
Publications in the MiPMap
Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biol 10:65-77. https://doi.org/10.1016/j.redox.2016.09.009

Β» PMID: 27693859 Open Access

Tyrrell Daniel J, Bharadwaj Manish S, Jorgensen Matthew J, Register Thomas C, Molina Anthony JA (2016) Redox Biol

Abstract: Blood based bioenergetic profiling strategies are emerging as potential reporters of systemic mitochondrial function; however, the extent to which these measures reflect the bioenergetic capacity of other tissues is not known. The premise of this work is that highly metabolically active tissues, such as skeletal and cardiac muscle, are susceptible to differences in systemic bioenergetic capacity. Therefore, we tested whether the respiratory capacity of blood cells, monocytes and platelets, are related to contemporaneous respirometric assessments of skeletal and cardiac muscle mitochondria. 18 female vervet/African green monkeys (Chlorocebus aethiops sabaeus) of varying age and metabolic status were examined for this study. Monocyte and platelet maximal capacity correlated with maximal oxidative phosphorylation capacity of permeabilized skeletal muscle (R=0.75, 95% confidence interval [CI]: 0.38-0.97; R=0.51, 95%CI: 0.05-0.81; respectively), isolated skeletal muscle mitochondrial respiratory control ratio (RCR; R=0.70, 95%CI: 0.35-0.89; R=0.64, 95%CI: 0.23-0.98; respectively), and isolated cardiac muscle mitochondrial RCR (R=0.55, 95%CI: 0.22-0.86; R=0.58, 95%CI: 0.22-0.85; respectively). These results suggest that blood based bioenergetic profiling may be used to report on the bioenergetic capacity of muscle tissues. Blood cell respirometry represents an attractive alternative to tissue based assessments of mitochondrial function in human studies based on ease of access and the minimal participant burden required by these measures.

Copyright Β© 2016 The Authors. Published by Elsevier B.V. All rights reserved. β€’ Keywords: Bioenergetics, Blood cells, Cellular respiration, Mitochondria, Muscle, PBMC, Buffer z, Blebbistatin

β€’ O2k-Network Lab: US NC Winston-Salem Molina AJA

Labels: MiParea: Respiration 

Organism: Human, Other mammals  Tissue;cell: Skeletal muscle, Blood cells, Platelet  Preparation: Permeabilized tissue, Isolated mitochondria, Intact cells 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, NS, ROX  HRR: Oxygraph-2k 

2016-11, MitoEAGLE blood cells reviews 

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