MiPNet23.04 IOC133 Innsbruck AT

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MiPNet23.04 IOC133 Innsbruck AT

Publications in the MiPMap
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Innsbruck AT, 2018 Sep 05-07. MitoFit Coaching Days - Basic IOC 133.

»

Oroboros (2018-09-05) Mitochondr Physiol Network

Abstract:
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MitoFit Coaching Days - Basic. Innsbruck, Austria; 2018 Sep 05-07.


O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit




General information

MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.

Venue

» Information on travel and venue: IOC Innsbruck.
» Hotel suggestion: Hotel Weisses Kreuz

Registration

  • closed

Tutors, lecturers, and managers


Programme

Tuesday 4th Topic Room
17:00 - 18:00 Welcome - Get-together: Introduction of participants and their research interests MiPArt
Wednesday 5th Topic Room
09:00 OroboPOS service and O2k instrumental setup - overview with videoclips ORO-MitoFit Lab
09:30 Hands-on: OroboPOS service and O2k instrumental setup ORO-MitoFit Lab
10:10 Hands-on: DatLab 7 ORO-MitoFit Lab
10:30 Coffee
11:00 Instrumental quality control 1: oxygen calibration ORO-MitoFit Lab
11:30 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on:Instrumental quality control 1: oxygen calibration (continuation)

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips ORO-MitoFit Lab
14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background

DL-Protocol: Instrumental O2 background TiP2k

ORO-MitoFit Lab
15:00 Coffee
15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Thursday 6th Topic Room
09:00 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
09:15 Application of the O2k Series H ORO-MitoFit Lab
09:30 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 ORO-MitoFit Lab
10:00 Coffee
10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.

DL-Protocol: SUIT-1_O2_pce_D03.DLP

DL-Protocol: SUIT-2 O2 pce D07.DLP

ORO-MitoFit Lab
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
13:30 Introduction to DatLab analysis ORO-MitoFit Lab
15:00 Coffee
15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Friday 7th Topic Room
09:00 MitoFit proficiency test:

Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
10:00 Coffee
10:30 MitoFit proficiency test:

Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.

DL-Protocol: SUIT-1_O2_pce_D03.DLP

DL-Protocol: SUIT-2 O2 pce D07.DLP

ORO-MitoFit Lab
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
13:15 DatLab analysis ORO-MitoFit Lab
14:00 DatLab analysis summary ORO-MitoFit Lab
15:00 Coffee
15:30 The Bioblast wiki, O2k-Network and MITOEAGLE MiPArt
16:15 Feedback & conclusions MiPArt
17:00 Farewell activity MiPArt


Participants

Participant Institution
Bergmeister Lisa Bergmeister Lisa AT_Innsbruck_Schwarzer C - Medical University, Innsbruck (AT)
Participant Daltro Cardoso Luiza Helena AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
Participant Roy Chowdhury Subir CA Winnipeg Banerji V - University of Manitoba, Winnipeg (CA)
Participant Gallee Leon AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
Garipi Enis Garipi Enis AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
Participant Huete-Ortega Maria AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
Participant Neufang Nora Sophie DE Hamburg Neufang NS - Universitätsklinikum Eppendorf, Hamburg (DE)
Perez Valencia Juan Alberto Perez Valencia Juan Alberto AT Innsbruck Oroboros - Leopold-Franzens-University, Innsbruck (AT)


Contact

instruments@oroboros.at


Oroboros Instruments
High-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Sponsors.png SPIN

IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.


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HRR: Oxygraph-2k 

ORO, IOC, MitoFit, SPIN, 2018, MitoGlobal