MiPNet23.04 IOC133 Innsbruck AT
 Innsbruck AT, 2018 Sep 05-07. MitoFit Coaching Days - Basic IOC 133. |
Oroboros (2018-09-05) Mitochondr Physiol Network
Abstract:
MitoFit Coaching Days - Basic. Innsbruck, Austria; 2018 Sep 05-07.
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
Venue
- » Information on travel and venue: IOC Innsbruck.
- » Hotel suggestion: Hotel Weisses Kreuz
Registration
- closed
Tutors, lecturers, and managers
Carolina Doerrier, PhD., Oroboros Instruments - Chief scientific officer
Timea Komlòdi, PhD., Oroboros Instruments - Scientific researcher
Manuela Passrugger, BSc., Oroboros Instruments - Biomedical Assistant
Marco di Marcello, BSc., Medical University of Innsbruck, MitoFit project - Assistant
Erich Gnaiger, PhD., Oroboros Instruments, CEO
Programme
Tuesday 4th Topic Room 17:00 - 18:00 Welcome - Get-together: Introduction of participants and their research interests MiPArt
Wednesday 5th Topic Room 09:00 OroboPOS service and O2k instrumental setup - overview with videoclips ORO-MitoFit Lab 09:30 Hands-on: OroboPOS service and O2k instrumental setup ORO-MitoFit Lab 10:10 Hands-on: DatLab 7 ORO-MitoFit Lab 10:30 Coffee 11:00 Instrumental quality control 1: oxygen calibration ORO-MitoFit Lab 11:30 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on:Instrumental quality control 1: oxygen calibration (continuation) DL-Protocol: O2 calibration air
ORO-MitoFit Lab 13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips ORO-MitoFit Lab 14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background DL-Protocol: Instrumental O2 background TiP2k
ORO-MitoFit Lab 15:00 Coffee 15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Thursday 6th Topic Room 09:00 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 09:15 Application of the O2k Series H ORO-MitoFit Lab 09:30 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 ORO-MitoFit Lab 10:00 Coffee 10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
ORO-MitoFit Lab 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 13:30 Introduction to DatLab analysis ORO-MitoFit Lab 15:00 Coffee 15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Friday 7th Topic Room 09:00 MitoFit proficiency test: Hands-on: Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 10:00 Coffee 10:30 MitoFit proficiency test: Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
ORO-MitoFit Lab 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 13:15 DatLab analysis ORO-MitoFit Lab 14:00 DatLab analysis summary ORO-MitoFit Lab 15:00 Coffee 15:30 The Bioblast wiki, O2k-Network and MITOEAGLE MiPArt 16:15 Feedback & conclusions MiPArt 17:00 Farewell activity MiPArt
Participants
| Participant | Institution | |
|---|---|---|
|
Bergmeister Lisa | AT_Innsbruck_Schwarzer C - Medical University, Innsbruck (AT) |
|
Daltro Cardoso Luiza Helena | AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT) |
|
Roy Chowdhury Subir | CA Winnipeg Banerji V - University of Manitoba, Winnipeg (CA) |
|
Gallee Leon | AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT) |
| Garipi Enis | AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT) | |
| Participant | Huete-Ortega Maria | AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT) |
|
|
Neufang Nora Sophie | DE Hamburg Neufang NS - Universitätsklinikum Eppendorf, Hamburg (DE) |
|
Perez Valencia Juan Alberto | AT Innsbruck Oroboros - Leopold-Franzens-University, Innsbruck (AT) |
Contact
- Oroboros Instruments
- High-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
HRR: Oxygraph-2k
ORO, IOC, MitoFit, SPIN, 2018, MitoGlobal
