Krumschnabel 2000a J Exp Biol

From Bioblast
Publications in the MiPMap
Krumschnabel G, Schwarzbaum PJ, Lisch J, Biasi C, Wieser W (2000a) Oxygen-dependent energetics of anoxia-tolerant and anoxia-intolerant hepatocytes. J Exp Biol 203:951-959.

Β» PMID:10667979 Open Access

Krumschnabel G, Schwarzbaum PJ, Lisch J, Biasi C, Wieser W (2000a) J Exp Biol

Abstract: The oxygen-dependence of cellular energetics was investigated in hepatocytes from goldfish Carassius auratus (anoxia-tolerant) and rainbow trout Oncorhynchus mykiss (anoxia-intolerant). In goldfish hepatocytes, an approximately 50% reduction in the rate of oxygen consumption was observed in response to both acute and prolonged hypoxia, the latter treatment shifting the threshold for this reduction to a higher oxygen level. A concomitant increase in the rate of lactate production did not compensate for the decreased aerobic ATP supply, resulting in an overall metabolic depression of 26% during acute hypoxia and of 42% during prolonged hypoxia. Trout hepatocytes showed a similar suppression of cellular respiration after prolonged hypoxia but were unresponsive to acute hypoxia. Similarly, the rate of lactate production was unaltered during acute hypoxia but was increased during prolonged hypoxia, metabolic depression amounting to 7% during acute hypoxia and 30% during prolonged hypoxia. In both species, the affinity of hepatocytes for oxygen decreased during hypoxia, but this alteration was not sufficient in absolute terms to account for the observed decrease in aerobic ATP supply. Protein synthesis was suppressed in both cell types under hypoxia, whereas Na+K+-ATPase activity decreased in trout but not in goldfish hepatocytes, emphasising the importance of membrane function in these cells during conditions of limited energy supply. β€’ Keywords: hypoxia, metabolic depression, protein synthesis, Na+/K+-ATPase, goldfish, Carassius auratus, rainbow trout, Oncorhynchus mykiss

β€’ O2k-Network Lab: AT Innsbruck Oroboros


Labels:

Stress:Ischemia-reperfusion  Organism: Fishes  Tissue;cell: Liver  Preparation: Intact cells 

Regulation: Oxygen kinetics 


HRR: Oxygraph-2k 

Environmental Physiology 

Cookies help us deliver our services. By using our services, you agree to our use of cookies.