Kramer 2019 Abstract IOC141

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Kramer P, Distefano G, Monte J, Mills A, Edmunds L, Jurczak M, Cummings SR, Kritchevsky S, Newman AB, Hepple RT, Goodpaster B, Coen PM, Molina A (2019) The Study of Muscle, Mobility, and Aging (SOMMA): A multi-institutional respirometry study. Mitochondr Physiol Network 24.02.

Link: IOC141

Kramer P, Distefano G, Monte J, Mills A, Edmunds L, Jurczak M, Cummings SR, Kritchevsky S, Newman AB, Hepple RT, Goodpaster B, Coen PM, Molina A (2019)

Event: IOC141

The decline in muscle mass and function with age can result in significant losses to mobility, independence and quality of life pose a significant financial burden to older adults. The cause of age-related loss of muscle mass and function are not adequately defined but may be due to a decreased mitochondrial efficiency and capacity for ATP production. The Study of Muscle, Mobility, and Aging (SOMMA) is a prospective, observational multi-institutional study that began enrollment in May 2019. The goal of the study is to recruit 875 older adults (>70 YOA) who are at risk of mobility disability at the University of Pittsburgh, and Wake Forest University. We will then track loss of mobility over a 3 year follow up period. Muscle biopsies are being collected at baseline to measure mitochondrial function. We hypothesize that mitochondrial function will predict slowing and incidence of mobility disability. As of August 2019, >90 respirometry assays have been performed, and internal and external quality control measures have been implemented between the two institutions. Quality control methods include muscle wet weight normalization, cytochrome c injection for outer membrane integrity, and assay timing data to account for variations in processing, permeabilization, and assay start time. The primary respirometry protocol consists of serial injections of pyruvate, malate, sub-saturating ADP (x2), saturating ADP, cytochrome c, glutamate, succinate, FCCP (x4), Antimycin A, and ascorbate/TMPD. The samples are run in duplicate on the Oroboros High-Resolution Respirometer. Assessing the mitochondrial function in muscle from older adults will provide valuable insight into the loss of muscle mass and mobility with age, and quality control measures will ensure reliable respirometry profiles between clinical sites.


Bioblast editor: Plangger M O2k-Network Lab: US FL Orlando Goodpaster BH, US FL Gainesville Hepple RT, US NC Winston-Salem Molina AJA


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style  Pathology: Aging;senescence 

Organism: Human  Tissue;cell: Skeletal muscle 


Coupling state: LEAK, OXPHOS, ET  Pathway: N, CIV, NS, ROX  HRR: Oxygraph-2k 


Affiliations

Kramer P(1), Distefano G(5), Monte J(2), Mills A(2), Edmunds L(2), Jurczak M(2), Cummings SR(4), Kritchevsky S(1), Newman AB(2), Hepple RT(3), Goodpaster B(5), Coen PM(5), Molina A(1)
  1. Wake Forest Univ, Winston-Salem, NC
  2. Univ Pittsburgh, Pittsburgh, PA
  3. Univ Florida, Gainesville, FL
  4. Univ California San Francisco, San Francisco, CA
  5. AdventHealth Translational Research Inst Metabolism and Diabetes, Orlando, FL; USA