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Jagendorf 1966 Proc Natl Acad Sci USA

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Publications in the MiPMap
Jagendorf AT, Uribe E (1966) ATP formation caused by acid-base transition of spinach chloroplasts. Proc Natl Acad Sci USA 55:170-7.

» PMID: 5220864 Open Access; PDF

Jagendorf AT, Uribe E (1966) Proc Natl Acad Sci USA

Abstract: Chloroplasts form a limited amount of ATP without illumination or oxygen if made first acid, then basic. The yields are greatly increased by having an appropriate organic acid present in the acid stage. Lack of specificity of the acid suggests it does not serve as a substrate for a specific enzyme. The highest yields obtained regularly have been 1 ATP for every 4 chlorophylls (i.e., 100 ATP per cytochrome f, or 40 µmoles ATP per gram of protein). Formation of ATP is sensitive to known uncouplers of photosynthetic phosphorylation, and the kinetics of either decay of the intermediate or of phosphorylation at pH 8 are the same as those for the high-energy condition induced by illumination at pH 6. The yield of ATP depends in part on the actual pH differential between the two experimental stages. The data are suggested to be consistent with a model in which the high energy condition consists of a pH gradient across the grana disk membranes. Keywords: ATP formation, Acid-base transition, Chloroplasts


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Organism: Plants 

Preparation: Chloroplasts 

Regulation: ADP, ATP production, Coupling efficiency;uncoupling, mt-Membrane potential, pH 



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