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Irving 2013 Abstract MiP2013

From Bioblast
Irving BA, Stahl R, Kane CJ, Chernousov M, Argyropoulos G, Carey D (2013) Sex differences in murine mitochondrial oxidative capacity following a 24 week high-fat diet. Mitochondr Physiol Network 18.08.


Brian Irving

MiP2013, Book of Abstracts Open Access

Irving BA, Stahl R, Kane CJ, Chernousov M, Argyropoulos G, Carey D (2013)

Event: MiPNet18.08_MiP2013

High-fat diets have been reported to affect skeletal muscle, white adipose tissue (WAT), and liver mitochondrial abundance and respiratory function in rodents [1,2]. However, few data exist that have examined sex differences in mitochondrial respiratory function in response to a high-fat diet in these metabolic tissues. We therefore examined whether there were differences in mitochondrial respiratory capacity between male and female Balbc mice following a 24 week of high-fat diet. High-resolution respirometry was utilized to perform a multiple substrate-inhibitor titration (SUIT) protocol to assess mitochondrial respiratory function in permeabilized skeletal muscle, liver, and WAT from male and female Balbc mice [3,4]. The SUIT protocol investigated uncoupled (LEAK) respiration (glutamate and malate), oxygen phosphorylation (OXPHOS) capacity (OXPHOSGM: glutamate, malate plus ADP; OXPHOSGMS: glutamate, malate, succinate, plus ADP), and electron transfer-pathway (ET-pathway) capacity (glutamate, malate, succinate, ADP, plus FCCP). Tibialis anterior, soleus, and liver samples were initially mechanically permeabilized with the Shredder SG3 followed by chemical permeabilization with digitonin to ensure complete permeabilization. WAT was minced and chemically permeabilized with digitonin. Overall, there were no significant differences between males and females for the amount of weight gained following the 24 week high-fat diet. In the tibialis anterior, the males had higher LEAK respiration (P<0.01), OXPHOSGM capacity (P<0.05), and OXPHOSGMS capacity (P<0.05) than the females. In the soleus, the males had higher LEAK respiration (P<0.01) than the females. In the WAT, the males had higher OXPHOSGMS (P<0.05) and ET capacity (P<0.05) than the females. In the liver, no significant differences were observed between males and females. In conclusion, in response to a high-fat diet males compared to females had higher skeletal muscle LEAK respiration in the tibialis anterior and soleus, higher OXPHOS capacity in the tibialias anterior and WAT, and higher ET capacity in WAT.

β€’ O2k-Network Lab: US PA Danville Irving BA, US LA Baton Rouge Irving BA

Labels: MiParea: Respiration, Comparative MiP;environmental MiP, Gender, Exercise physiology;nutrition;life style  Pathology: Obesity 

Organism: Mouse  Tissue;cell: Skeletal muscle, Liver, Fat  Preparation: Permeabilized tissue, Homogenate 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, NS  HRR: Oxygraph-2k 

MiP2013, PBI-Shredder 

Affiliations and author contributions

1 - Geisinger Obesity Institute;

2 - Weis Center for Research, Geisinger Medical Center, Danville, PA, USA.

Email: [email protected]


  1. Iossa S, Lionetti L, Mollica MP, Crescenzo R, Botta M, Barletta A, Liverini G (2003) Effect of high-fat feeding on metabolic efficiency and mitochondrial oxidative capacity in adult rats. British J Nutrition 90: 953-960.
  2. Rong JX, Qiu Y, Hansen MK, Zhu L, Zhang V, Xie M, Okamoto Y, Mattie MD, Higashiyama H, Asano S, Strum JC, Ryan TE (2007) Adipose mitochondrial biogenesis is suppressed in db/db and high-fat diet-fed mice and improved by rosiglitazone. Diabetes 56: 1751-1760.
  3. Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain, and liver. Mitochondr Physiol Network 17.15: 1-6.
  4. Kraunsoe R, Boushel R, Hansen CN, Schjerling P, Qvortrup K, Stockel M, Mikines KJ, Dela F (2010) Mitochondrial respiration in subcutaneous and visceral adipose tissue from patients with morbid obesity. J Physiol 588: 2023-2032.