Gvozdjakova 2020 Diagnostics (Basel)

Gvozdjáková A, Sumbalová Z, Kucharská J, Komlósi M, Rausová Z, Vančová O, Számošová M, Mojto V (2020) Platelet mitochondrial respiration, endogenous coenzyme Q₁₀ and oxidative stress in patients with chronic kidney disease. Diagnostics (Basel) 10:176.

» PMID: 32210203 Open Access »

Gvozdjakova Anna, Sumbalova Zuzana, Kucharska Jarmila, Komlosi Maria, Rausova Zuzana, Vancova Olga, Szamosova Monika, Mojto Viliam (2020) Diagnostics (Basel)

Abstract: Chronic kidney disease (CKD) is characterized by a progressive loss of renal function and a decrease of glomerular filtration rate. Reduced mitochondrial function, coenzyme Q₁₀ (CoQ₁₀), and increased oxidative stress in patients with CKD contribute to the disease progression. We tested whether CoQ₁₀ levels, oxidative stress and platelet mitochondrial bioenergetic function differ between groups of CKD patients.

Twenty-seven CKD patients were enrolled in this trial, 17 patients had arterial hypertension (AH) and 10 patients had arterial hypertension and diabetes mellitus (AH and DM). The control group consisted of 12 volunteers. A high-resolution respirometry (HRR) method was used for the analysis of mitochondrial bioenergetics in platelets, and an HPLC method with UV detection was used for CoQ₁₀ determination in platelets, blood, and plasma. Oxidative stress was determined as thiobarbituric acid reactive substances (TBARS).

Platelets mitochondrial respiration showed slight, not significant differences between the groups of CKD patients and control subjects. The oxygen consumption by intact platelets positively correlated with the concentration of CoQ₁₀ in the platelets of CKD patients.

A decreased concentration of CoQ₁₀ and oxidative stress could contribute to the progression of renal dysfunction in CKD patients. The parameters of platelet respiration assessed by high-resolution respirometry can be used only as a weak biological marker for mitochondrial diagnosis and therapy monitoring in CKD patients. • Keywords: Chronic kidney disease, Coenzyme Q10, Citochondria, Oxidative stress, Platelets, Respiration • Bioblast editor: Plangger M • O2k-Network Lab: SK Bratislava Sumbalova Z

Cited by

Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003

Labels: MiParea: Respiration, Patients Pathology: Other

Organism: Human Tissue;cell: Platelet Preparation: Permeabilized cells, Intact cells

Coupling state: LEAK, ROUTINE, OXPHOS, ET Pathway: N, S, NS, ROX HRR: Oxygraph-2k

2020-04, O2k-brief, MitoFit 2021 PLT, MitoFit 2021 CoQ