Dudzinska 2012 Abstract Bioblast

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Dudzinska D, Luzak B, Stanczyk L, Rywaniak J, Watala C (2012) The influence of the extract from Rubus parvifolius leaves (EL17) on expression and activity of apyrase (CD 39) on human umbilical vein endothelial cells (HUVECs). Mitochondr Physiol Network 17.12.

Link: MiPNet17.12 Bioblast 2012 - Open Access

Dudzinska D, Luzak B, Stanczyk L, Rywaniak J, Watala C (2012)

Event: Bioblast 2012

Dominika Dudzinska

Human Endothelial Cells possess a lot of mechanisms which enable them prevention against thrombosis and plaque formation. One of such preventive systems is antigen CD39 which has the ability to decompose ATP and ADP. This process results in decreased aggregation of blood platelets activated via purinergic pathway [1]. Polyphenols, plant secondary metabolites, are widely known for their antioxidative and cardioprotective activities [2]. Blackberry fruits, which are the rich source of polyphenolic compounds, were proved in the in vivo studies to possess antithrombotic properties. We examined the influence of polyphenolic extract derived from Rubus parvifolius leaves (EL17), on the expression and ADPase activity of CD39 in HUVECs and on blood platelet aggregation. Platelet aggregation was determined in the whole blood obtained from healthy individuals. Whole blood samples were incubated with EL17 at the concentration of 7.5 and 15 µg/ml and agonized with 6.4 µg/ml ADP. To assess the effect on CD39 expression, HUVECs were incubated for 24 h with EL17 (at the concentration of 1, 5, 10 or 15 µg gallic acid per ml), then labeled witch anti-CD39 antibodies, washed (two times) and measured by flow cytometry. To estimate CD39 ADPase activity HUVECs were incubated with EL17 for 24 h and the samples were assayed using Malachite Green method. Platelet aggregation, upon the incubation with 15 µg/ml EL17, was inhibited by 19% (132 ± 53 AU vs. 165 ± 52 AU in control without EL17). Moreover, the expression of CD 39 increased significantly after incubation with EL17 (up to 30% and 50% after the treatment with 10 and 15 µg/ml EL17, respectively). The ADPase activity in the presence of EL17 remained at the same level as in control. To conclude, the extract from Rubus parvifolius leaves, exerts beneficial influence on platelet reactivity via purinergic pathway and may be considered as a potential antiplatelet modulator in antiplatelet therapy and prevention of cardiovascular diseases.

  1. Dauchet L, Amouyel P, Hercberg S, Dallongeville J (2006) Fruit and vegetable consumption and risk of coronary heart disease: a meta-analysis of cohort studies. J Nutr 136: 2588-2593. Open Access
  2. Marcus AJ, Broekman MJ, Drosopoulos JH, Islam N, Alyonycheva TN, Safier LB, Hajjar KA, Posnett DN, Schoenborn MA, Schooley KA, Gayle RB, Maliszewski CR (1997) The endothelial cell ecto-ADPase responsible for inhibition of platelet function is CD39. J Clin Invest 99: 1351-1360. Open Access

Keywords: Polyphenols, HUVEC, Platelet aggregation

O2k-Network Lab: PL Lodz Watala C


Tissue;cell: HUVEC 

Affiliations and author contributions

Dominika Dudzińska, Bogusława Luzak, Lidia Stańczyk, Joanna Rywaniak, Cezary Watała

Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Lodz, Poland; Email: dominika.dudzinska@umed.lodz.pl

The poster is supported by the project “Production of polyphenol extracts of plant origin with antiplatelet and cardioprotective properties – FLAWOPIRYNA”, co-financed by European Union from European Regional Development Fund within the frames Innovative Economy Operational Programme.

Figure 1

Effect of EL17 on CD39 expression on HUVEC’s surface

Effect of EL17 on CD39 expression on HUVEC’s surface. Data shown as median and interquartile range (n=14). At higher concentration EL17 increased CD39 expression