Charles 2020 Nanomedicine (Lond)
|Charles C, Cohen-Erez I, Kazaoka B, Melnikov O, Stein DE, Sensenig R, Rapaport H, Orynbayeva Z (2020) Mitochondrial responses to organelle-specific drug delivering nanoparticles composed of polypeptide and peptide complexes. Nanomedicine (Lond) 15:2917-32.|
Abstract: The mechanistic study of the drug carrier-target interactions of mitochondria-unique nanoparticles composed of polypeptide-peptide complexes (mPoP-NPs).
The isolated organelles were employed to address the direct effects of mPoP-NPs on dynamic structure and functional wellbeing of mitochondria. Mitochondria morphology, respiration, membrane potential, reactive oxygen species generation, were examined by confocal microscopy, flow cytometry and oxygraphy. Lonidamine-encapsulated formulation was assessed to evaluate the drug delivery capacity of the naive nanoparticles.
The mPoP-NPs do not alter mitochondria structure and performance upon docking to organelles, while successfully delivering drug that causes organelle dysfunction.
The study gives insight into interactions of mPoP-NPs with mitochondria and provides substantial support for consideration of designed nanoparticles as biocompatible and efficient mitochondria-targeted platforms. • Keywords: OxPhos, TPP+, Cancer, Ionidamine, Membrane potential, Mitochondria, Nanoparticles • Bioblast editor: Plangger M • O2k-Network Lab: US PA Philadelphia Orynbayeva Z
Labels: MiParea: Respiration, mt-Structure;fission;fusion, Pharmacology;toxicology
Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell Preparation: Isolated mitochondria
Regulation: mt-Membrane potential Coupling state: LEAK, OXPHOS Pathway: N, S, CIV, ROX HRR: Oxygraph-2k, O2k-Fluorometer, TPP