Bioblast alert 2011

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Bioblast alert 2011

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Bioblast alert

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Bioblast alert 2011(01): 2011-11-11

  • A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?
Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.
  • ET-capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations.
  • Basal respiration - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology.


Bioblast alert 2011(02): 2011-11-17

  • Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.
Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738. --> Discussion: Complementary results on the PGC-1a pathway.
  • Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols.
Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.


Bioblast alert 2011(03): 2011-11-24

  • Fluorometry combined with high-resolution respirometry: Light guide inserted into the chamber through a black PEEK stopper[1], or optical probe operated through the O2k-glass window[2].
  1. Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300:H118-24.
  2. Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182:91-100.

Bioblast alert 2011(04): 2011-12-24

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58.