Talk:Bioblast alert 2015

Bioblast alert - in preparation

Bioblast alert 2012 (6): 2012-x-x

MitoPedia topic: Autoxidation: Ascorbate - TMPD --> Autoxidation - DEFINE!!

Bioblast news:

* OPEN for registration: 48th Annual Meeting EASD - European Association for the Study of Diabetes - 2012 Oct 1-5, Berlin, Germany.

* 5th MiPsummer School on Mitochondrial Physiology FEBS Workshop 2012 Jul 6-12, Cambridge, UK - Programme - Organizer: Guy Brown - www.mitophysiology.org

Highlighted MiPNet Reference Laboratories

• DE Duesseldorf Roden M * US MN Rochester Nair KS * UK Cambridge Brown GC

Bioblast alert 2012 (5): 2012-06-04

Comparison of common bioenergetic analysis methods: Zhang J, Nuebel E, Wisidagama DR, Setoguchi K, Hong JS, Van Horn CM, Imam SS, Vergnes L, Malone CS, Koehler CM, Teitell MA (2012) Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells Nat Protoc 7: 1068-1085.

The O2k in cell culture apllications:

• Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.
• Garedew A, Haffner B, Huetter E, Gnaiger E.An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04.
• Gnaiger E.Isolated mitochondria or permeabilized tissues and cells. Mitochondr Physiol Network 11.05.

* Also see: Mitochondrial preparations - Cultured cells

• OROBOROS vs. Seahorse - continue the DISCUSSION

Term of the week

• Peroxisome proliferator-activated receptor-γ (PPAR-γ) coactivator-1α

• UMDF: Mitochondrial Medicine 2012 - 2012-June-13 to 16 - Capitol Hill, Bethesda, Washington DC, USA

Bioblast alert 2012 (##): 2012-##-##

Mitochondrial dys/function in heart vs. brain:

1. Wüst RC, Myers DS, Stones R, Benoist D, Robinson PA, Boyle JP, Peers C, White E, Rossiter HB (2011) Regional skeletal muscle remodeling and mitochondrial dysfunction in right ventricular heart failure. Am J Physiol Heart Circ Physiol. 302: H402-11. Wuest 2012 Am J Physiol Heart Circ Physiol.
2. Boyle JP, Hettiarachchi NT, Wilkinson JA, Pearson HA, Scragg JL, Lendon C, Al-Owais MM, Kim CB, Myers DM, Warburton P, Peers C (2012) Cellular consequences of the expression of Alzheimer's disease-causing presenilin 1 mutations in human neuroblastoma (SH-SY5Y) cells. Brain Res Epub ahead of print. Boyle 2012 Brain Res.

MiPNet Laboratory of the week

• UK: Prof. Dr. Chris Peers and Dr. John Boyle

Bioblast alert ### to select

• Scientific service: Oxygen regime for permeabilized fibres vs. isolated mitochondria - reoxygenations in MiR06.
• Various chemicals:

Carboxyatractyloside vs. atractyloside

Cat is considered to be much more specific than Atractyloside (Atr), and the effective concentration required is much much lower (µM). With Atr, a very high concentration (mM) is required for full inhibition.

Gnaiger E: When we used atractyloside (Atr), carboxyatractyloside (Cat) was off the market, hence we had no other choice. Cat is available again. Even if it costs more, it is worth more, and with Cat available, I would not bother to find out more about Atr.

Chinopoulos C: I have used the Sigma Cat as well, but not for titration studies. It works perfectly at 1-2 µM concentrations, if you want to block completely all ANTs. The MW is 770.8.

The Sigma Cat originates from Atr extracted from Xanthicum sibericum. A carboxylation is a further chemical modification. The Merck Cat originates from Atr from Atractylis gummifera, which is identical to that from Xanthicum. A carboxylation is a further chemical modification. Because of this modification which is man-made, there may be different potencies. For titration studies (0-200 nM Cat), the one from Merck (Atractylis gummifera) is strongly recommended. - Christos (2012-04-19)

Chu MJJ: Unfortunately Merck does not sell the Cat from Atractylis gummifera anymore and currently only offers the Cat extracted from Xanthicum sibericum (as does Sigma). - Michael (2012-04-20)

Alpha-glycerophosphate

DL-alpha-glycerophosphate disodium salt hexahydrate (FW= 324.1), Sigma G-2138

Sigma is not selling alpha-glycerophosphate any more!

Myxothiazol

Myxothiazol is again available from Sigma, but with the new product number T5580. The old product number was M 5779.

Bioblast alert - concept

Here is a brief summary on the concept of Bioblast alert.

Some of the following topics (not more than four per alert) will be selected for a Bioblast alert.

MiPNet O2k-Publications

• Topic: O2-kinetics; coupling; pathology; ...
• Preparation: Homogenate/isolated mitochondria; intact/permeabilized cells; permeabilized fibre/in vivo
• Protocols

MiPNet Laboratories of the World

Contact the MiPNet laboratories: show link on the wiki, ask for updates on group, references, website, perhaps major projects (HypoxiaNet), perhaps add pictures, perhaps keywords/short description. Circular: link to presented lab / special occasion (e.g. publication; conference presentation; new member; PhD thesis, ..

High-resolution terminology

• high-resolution terminology - matching measurement at high-resolution. Term of the week: Basal respiration
• Define – Measure: terminology and experimental approach
• Define – Clarify: consider discrepancies, resolve problems

MiPevents

• Conferences and Workshops

Bioblast

• What is new or updated on the website: Lists (chemicals, protocols, scientific service, ..)
• Projects in mitochondrial science (COST HypoxiaNet; Framework programs; ..)
• Links on the Bioblast website; networking in mitochondrial science, presentation of: MIG List, Mitochondrion journal and website; Mitochondrial Societies
• MitoCom News

Bioblast editors

A Bioblast editor may select an O2k-Publication (or a small number of related publications), particularly from a MiPNet Reference Laboratory, may get in contact with the particular reference lab, update info on the lab, and ‘present’ the lab in the alert. A term may be selected (perhaps related to the publication), updated, discussed in our group, and then presented in the alert. An editor does not even have to present an entire alert, but may prepare individual ‘points’, which then can be added to a particular alert. As Bioblast is updated, the updated ‘article’ (term, publication etc) can be added to the prepared points for a future alert.