Talk:Azide: Difference between revisions
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(deleted titration instructions, because they did not change by taking the volume of the solute into account) |
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== Former Instructions == | |||
These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended. | |||
::::'''Preparation of 4 M storage and stock solution''' (dissolved in H<sub>2</sub>O) for '''2-mL O2k-chamber''': | |||
::::# Weigh 260 mg of sodium azide. | |||
::::# Dissolve in 1 mL H<sub>2</sub>0. | |||
::::# Divide into 0.5 mL portions. | |||
::::# Store at -20 °C. | |||
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:::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en®ion=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; M = 65.01 g·mol<sup>-1</sup>. | :::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en®ion=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; M = 65.01 g·mol<sup>-1</sup>. | ||
:::: 2021-02: The preparation instructions were updated to take the volume of the solute ( | :::: 2021-02: The preparation instructions were updated to take the volume of the solute into account (see [[User:Spitzer_Gudrun/Volume_of_the_solute]]). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations. | ||
::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span> | ::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span> | ||
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::::* Titration volume ('''0.5-mL O2k-chamber'''): ≥25 µL using a 100 µL Hamilton syringe. | ::::* Titration volume ('''0.5-mL O2k-chamber'''): ≥25 µL using a 100 µL Hamilton syringe. | ||
::::* Final concentration: ≥100 mM. | ::::* Final concentration: ≥100 mM. | ||
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Latest revision as of 13:04, 8 March 2021
Former Instructions
These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended.
- Preparation of 4 M storage and stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 260 mg of sodium azide.
- Dissolve in 1 mL H20.
- Divide into 0.5 mL portions.
- Store at -20 °C.
