Azide

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MitoPedia

Azide

Description

Sodium azide is an inhbitor of cytochrome c oxidase (COX, CcO).

Abbreviation: Azd

Reference: MiPNet03.02



MitoPedia topics: Inhibitor 

Application in HRR

Azd: Azide (Sodium azide, NaN3); Sigma S2002, SDS 25 g, store commercial stock at RT; FW = 65.01.

Caution: Very toxic!


Preparation of 4 M storage and stock solution (dissolved in H20):

1) Weigh 260 mg of sodium azide.
2) Dissolve in 1 ml H20.
3) Divide into 0.5 ml portions.
4) Store at -20 °C.


O2k manual titrations MiPNet09.12 O2k-Titrations

  • Titration volume: ≥50 µl using a 50 µl syringe (2 ml O2k-chamber).
  • Final concentration: ≥100 mM.