Talk:Azide: Difference between revisions

Latest revision as of 13:04, 8 March 2021

Former Instructions

These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended.

Preparation of 4 M storage and stock solution (dissolved in H₂O) for 2-mL O2k-chamber:

Weigh 260 mg of sodium azide.
Dissolve in 1 mL H₂0.
Divide into 0.5 mL portions.
Store at -20 °C.

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+== Former Instructions ==
+These instructions were given until 2020-03-08. They were updated because the volume of the solute was not taken into account. Therefore the concentration oft the stock solution was lower than intended.
+::::'''Preparation of 4 M storage and stock solution''' (dissolved in H<sub>2</sub>O)  for '''2-mL O2k-chamber''':
+::::# Weigh 260 mg of sodium azide.
+::::# Dissolve in 1 mL H<sub>2</sub>0.
+::::# Divide into 0.5 mL portions.
+::::# Store at -20 °C.
+<!--
 [[Image:BB-Bioblast.jpg|left|40px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]]
 == Popular Bioblast page ==
 ::: [[Azide]] has been accessed more than
 ::::*  5,000 times (2016-05-06)
+== Application in [[HRR]] (updated instructions) ==
+:::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en&region=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; M = 65.01 g·mol<sup>-1</sup>.
+:::: 2021-02: The preparation instructions were updated to take the volume of the solute into account (see [[User:Spitzer_Gudrun/Volume_of_the_solute]]). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.
+::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span>
+::::'''Preparation of 4 M storage and stock solution''' (1 mL, dissolved in H<sub>2</sub>O)  for '''2-mL O2k-chamber''':
+::::# Weigh 260 mg of sodium azide.
+::::# Dissolve in 900 µL H<sub>2</sub>0.
+::::# Divide into 0.5 mL portions.
+::::# Store at -20 °C.
+:::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+::::* Titration volume ('''2-mL O2k-chamber'''): ≥50 µL using a 100 µL Hamilton syringe.
+::::* Final concentration: ≥100 mM.
+::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O)  for '''0.5-mL O2k-chamber''':
+::::# Take 400 µL of the 4 M stock solution.
+::::# Add 400 µL H<sub>2</sub>O.
+:::»'''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+::::* Titration volume ('''0.5-mL O2k-chamber'''): ≥25 µL using a 100 µL Hamilton syringe.
+::::* Final concentration: ≥100 mM.
+-->