Talk:Azide

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Application in HRR (updated instructions)

Azd: Azide (Sodium azide, NaN₃); Sigma S2002, SDS 25 g, store commercial stock at RT; M = 65.01 g·mol^-1.

2021-02: The preparation instructions were updated to take the volume of the solute into account. The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.

Caution: Very toxic!

Preparation of 4 M storage and stock solution (1 mL, dissolved in H₂O) for 2-mL O2k-chamber:

1. Weigh 260 mg of sodium azide.
2. Dissolve in 900 µL H₂0.
3. Divide into 0.5 mL portions.
4. Store at -20 °C.

»O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (2-mL O2k-chamber): ≥50 µL using a 100 µL Hamilton syringe.
• Final concentration: ≥100 mM.

Preparation of 2 M stock solution (dissolved in H₂O) for 0.5-mL O2k-chamber:

1. Take 400 µL of the 4 M stock solution.
2. Add 400 µL H₂O.

»O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume (0.5-mL O2k-chamber): ≥25 µL using a 100 µL Hamilton syringe.
• Final concentration: ≥100 mM.