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Revision as of 12:19, 28 January 2019 by Komlodi Timea (talk | contribs) (Created page with "{{MitoPedia |abbr=NS(PGM) |description=300px|SUIT-020 |info='''A''': '''SUIT-020''' short protocol for simultaneous determination of O<s...")
- high-resolution terminology - matching measurements at high-resolution
SUIT-020 Fluo mt D033
Description
Abbreviation: NS(PGM)
Reference: A: SUIT-020 short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria and tissue homogenate).
SUIT number: D033_1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama
O2k-Application: AmR
- SUIT-category: NS(PGM)
- SUIT protocol pattern: orthogonal 1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama
Communicated by Komlodi T (last update 2019-01-28)
DLP applications
- SUIT-020 Fluo mt D033 simultaneous determination of O2 flux and mt-membrane potential using any fluorescence dye (Safranin, TMRM etc) on isolated mitochondria and tissue homogenate.
- SUIT-020 Fluo mt D036 simultaneous determination of O2 flux and mt-membrane potential using any fluorescence dye (Safranin, TMRM etc) on isolated mitochondria and tissue homogenate.
- SUIT-020 O2 mt D032 determination of O2 flux on isolated mitochondria and tissue homogenate as a control for SUIT-020 Safr mt D033.
- SUIT-020 O2 mt D035 determination of O2 flux on isolated mitochondria and tissue homogenate as a control for SUIT-020 Safr mt D036.
References
| Year | Reference | Organism | Tissue;cell | |
|---|---|---|---|---|
| MiPNet20.13 Safranin mt-membranepotential | 2019-06-24 |  | Mouse | Nervous system |
| Krumschnabel 2014 Methods Enzymol | 2014 | Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. https://doi.org/10.1016/B978-0-12-416618-9.00009-1 | Mouse | Nervous system |
Strengths and limitations
- + Information in one protocol about the NS, S and N-pathway control state.
- - The fluorescence dye (such as Safranin, TMRM etc) can inhibit most porbably the NADH-linked respiration, therefore, the protocol has to be run without using the fluorescence dye. The following protocols can be used: SUIT-020 O2 mt D032, SUIT-020 O2 mt D035.
- - CIV activity and Cytochrome c test cannot be performed together with the fluorescence.
Compare SUIT protocols
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Fluorometry
