SUIT-015 O2 pti D043

high-resolution terminology - matching measurements at high-resolution

SUIT-015 O2 pti D043

Description

Abbreviation: FNS(Oct,PGM)

Reference: A pti: permeabilized tissue- SUIT-015

SUIT number: D042_1OctM;2D;3G;4P;5S;6U;7Rot;8Ama

O2k-Application: O2

SUIT-category: FNS(Oct,PGM)

SUIT protocol pattern: diametral 1OctM;2D;3G;4P;5S;6U:7Rot-

Communicated by Iglesias-Gonzalez J and Gnaiger E (last update 2019-02-04)

Strengths and limitations

+ The protocol provides information on FAO capacity in the absence of other, potentially interfering pathways, both in the LEAK state and in OXPHOS.

+ FNS OXPHOS capacity comprises the most important pathways in many cell types and thus provides a physiologically relevant estimate of maximum mitochondrial respiratory capacity.

+ FNS ET capacity is a good estimate of overall ET capacity in many cell types.

+ The presence of PMG and S establishes a fully operative TCA cycle activity.

+ Application of the cytochrome c test early in the protocol ensures comparability of all states in case of any effect of c.

+ Reasonable duration of the experiment.

- F OXPHOS capacity may be underestimated. In human heart muscle addition of Oct to palmitoylcarnitine (Pal) + malate increased OXPHOS by 26% (Lemuieux et al 2011).

- SRot(E) may be underestimated if S is not saturating.

- CIV activity is not measured, to save experimental time.

Compare SUIT protocols

• SUIT-002
• SUIT-019
• 1PGM;2D;3S;4U;5Rot- 1PGM;2D;3S;4U;5Rot-

References

MitoPedia concepts: SUIT protocol, SUIT A, Find 

MitoPedia methods: Respirometry