SUIT-009 O2 mt D015: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
Line 9: | Line 9: | ||
::: '''[[SUIT protocol pattern]]:''' orthogonal 1S;2D;3P;4Rot | ::: '''[[SUIT protocol pattern]]:''' orthogonal 1S;2D;3P;4Rot | ||
__TOC__ | __TOC__ | ||
Communicated by [[Iglesias-Gonzalez J], [[Komlodi T]] and [[Gnaiger E]] (last update 2019-01-22) | Communicated by [[Iglesias-Gonzalez J]], [[Komlodi T]] and [[Gnaiger E]] (last update 2019-01-22) | ||
== SUIT protocols == | == SUIT protocols == | ||
::::* [[SUIT-009 AmR mt D021]] | ::::* [[SUIT-009 AmR mt D021]] |
Revision as of 17:18, 22 January 2019
Description
Abbreviation: NS(P)
Reference: A: SUIT-009 short protocol for respiration in mitochondrial preparations (isolated mitochondria and tissue homogenate).
SUIT number: D015_1S;2D;3P;4Rot;5Ama
- SUIT-category: NS(P)
- SUIT protocol pattern: orthogonal 1S;2D;3P;4Rot
Communicated by Iglesias-Gonzalez J, Komlodi T and Gnaiger E (last update 2019-01-22)
SUIT protocols
References
Strengths and limitations
- For H2O2-Application, choose SUIT-009 AmR mt D021; for O2 and H2O2 measurements (AmR), choose: SUIT-009 AmR mt D021.
- Cytochrome c test can be performed in the S-OXPHOS state.
- +Reasonable duration of the experiment.
- +This protocol can be extended with the Complex IV module.
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry