From Bioblast
Revision as of 17:10, 22 January 2019 by Cardoso Luiza (talk | contribs) (Created page with "{{MitoPedia |abbr=N(GM) |description=400px |info='''A''' |application=O2 |SUIT number=D030_ce1;1Dig;1G;2D;2c;3M;4U;5Ama }} ::: '''Categ...")
Description
Abbreviation: N(GM)
Reference: A
SUIT number: D030_ce1;1Dig;1G;2D;2c;3M;4U;5Ama
O2k-Application: O2
- SUIT-category: N(GM)
- SUIT protocol pattern: diametral ce1;1Dig;1G;2D;2c;3M;4U;5Ama
- Linked to
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
1Dig | REN | ce1;1Dig
|
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1G | GL | (N) | CI | 1G
|
2D | GP | (N) | CI | 1G;2D
|
2c | GcP | (N) | CI | 1G;2D;2c
|
3M | GMP | N | CI | 1G;2D;2c;3M
|
4U | GME | N | CI | 1G;2D;2c;3M;4U
|
5Ama | ROX | 1G;2D;2c;3M;4U;5Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- This protocol is indicated when the aim is to analyse coupling control in mtprep (see MitoPedia: Sample preparations) at Succinate pathway control state.
- + The combination of succinate with rotenone, that inhibits complex I, provides the best coupling control protocol specific for S-pathway, without any contribution of N- and F- pathways.
- + Short duration of the experiment.
- + CIV activity can be measured as an extension.
- - This protocol does not include internal ET-pathway control steps.
- - Careful washing is required after the experiment to avoid carry-over of uncoupler and inhibitors.
Compare SUIT protocols
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry