Difference between revisions of "SUIT-007"

Revision as of 11:01, 17 January 2019

high-resolution terminology - matching measurements at high-resolution

SUIT-007

Description

[[File:|300px]]

Reference: A: »Versions

SUIT-category:

SUIT protocol pattern:

DLP applications

References

Strengths and limitations

Compare SUIT protocols

MitoPedia concepts: MiP concept, SUIT protocol, Recommended

MitoPedia methods: Respirometry

@@ Line 1: / Line 1: @@
 {{MitoPedia
-|abbr=ceP,S
+|abbr=
-|description=[[File:Ce1;ce2P;ce3Omy;ce4U;ce5Rot;ce6S;1Dig;1U;1c-.png|300px]]
+|description=[[File:|300px]]
 |info='''A:''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-007.pdf|Bioblast pdf]] »[http://wiki.oroboros.at/index.php/File:SUIT-007.pdf Versions]
 }}
-::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceP,S
+::: '''[[Categories of SUIT protocols |SUIT-category]]:'''
-::: '''[[SUIT protocol pattern]]:''' complex cell [[coupling control protocol]] ce1;ce2P;ce3Omy;ce4U;ce5Rot;ce6S;1Dig;1c;2Ama
+::: '''[[SUIT protocol pattern]]:'''
 __TOC__
 == DLP applications ==
-::::* [[SUIT-003 O2 ce-pce D013]]
-::::* [[SUIT-003 O2 ce-pce D018]]
-::::* [[SUIT-003 O2 ce-pce D020]]
 == References ==
@@ Line 26: / Line 23: @@
 |order=descending
 }}
-{{Template:SUIT-007}}
+{{Template:}}
 == Strengths and limitations ==
-:::* For determination of respirometric viability index, it is required cells with functional mitochondria from both intact and permeabilized (dead) cell population.
-:::+ S-ET capacity can be assessed after the performance of the coupling control protocol (see [[SUIT-003]]). ce6S, 1Dig and 1c steps can be implemented without interfering other steps.
-:::+ Respirometric viability test correlates with other viability methods.
-:::+ CIV activity is measured.
-:::- Cells with succinate transporter are not suitable for the viability test.
-:::- Pathological profiles could hinder the viability test.
-:::- Longer protocol in comparison to [[SUIT-003]]. Protocol average length of 2 hours.
-:::- Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler.
 == Compare SUIT protocols ==
-::::*
 {{MitoPedia concepts