Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-"
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| 4ceU | | 4ceU | ||
| ''E''+S<sub>''E''</sub> | | ''E''+S<sub>''E''</sub> | ||
| [[ | | [[ET-pathway]] (''E'') + [[S]] (S<sub>''E''</sub>) | ||
| ce and dce are in the | | ce and dce are in the ET state. | ||
|- | |- | ||
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| ROX+S<sub>''E''</sub> | | ROX+S<sub>''E''</sub> | ||
| [[ROX]]+[[S]] (S<sub>''E''</sub>) | | [[ROX]]+[[S]] (S<sub>''E''</sub>) | ||
| ce are in the ROX state, dce are in the | | ce are in the ROX state, dce are in the ET state. | ||
|- | |- |
Revision as of 14:15, 20 October 2017
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
Abbreviation: ceRLE(SD)
Reference: A Stadlmann 2006 Cell Biochem Biophys
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: ceRLE(SD)
- SUIT protocol pattern: complex cell coupling control protocol (ceCCP)
Coupling control protocol
- 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma
Step | Respiratory state | Pathway control | Comment |
---|---|---|---|
1ce | ROUTINE | Intact cells (ce) | Dead cells (dce) without substrate are in a state of ROX. |
2ceSD | R+SP | ROUTINE (R) + S (SP) | dce with functional mitochondria are in state SP. |
3ceOmy | L+SL | LEAK (L) + S (SL) | ce and dce are in the LEAK state. |
4ceU | E+SE | ET-pathway (E) + S (SE) | ce and dce are in the ET state. |
5ceRot | ROX+SE | ROX+S (SE) | ce are in the ROX state, dce are in the ET state. |
6ceAma | ROXAma | ROX |
- 1ce;1ceS .. versus 1ce;2ceOmy;3ceU;4ceS .. and respiration medium
- Intact cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).