Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-"
From Bioblast
m (Gnaiger Erich moved page 1ce;2SD;3Omy;4U- to 1ce;2ceSD;3ceOmy;4ceU-) |
|||
| Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=RLE(SD) | |abbr=RLE(SD) | ||
|description=[[File: | |description=[[File:1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma.jpg|200px]] | ||
|info='''A''' [[Stadlmann 2006 Cell Biochem Biophys]] | |info='''A''' [[Stadlmann 2006 Cell Biochem Biophys]] | ||
}} | }} | ||
| Line 8: | Line 8: | ||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' RLE(SD) | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' RLE(SD) | ||
::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] ( | ::: '''[[SUIT protocol pattern]]:''' complex cell [[coupling control protocol]] (ceCCP) | ||
[[File:1R,2R(SD),3L(S),4E(S)-5ROX(S),6ROX.jpg|right|300px]] | [[File:1R,2R(SD),3L(S),4E(S)-5ROX(S),6ROX.jpg|right|300px]] | ||
[[File:Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg|500px]] | [[File:Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg|500px]] | ||
== Coupling control protocol == | == Coupling control protocol == | ||
::::* | ::::* 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma | ||
{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
| Line 23: | Line 23: | ||
|- | |- | ||
| | | 1ce | ||
| [[ROUTINE]] | | [[ROUTINE]] | ||
| [[Intact cells |ce]] | | [[Intact cells |Intact cells (ce)]] | ||
| | | Dead cells (dce) without substrate are in a state of ROX. | ||
|- | |- | ||
| | | 2ceSD | ||
| ''R''+S<sub>''P''</sub> | | ''R''+S<sub>''P''</sub> | ||
| [[ROUTINE]] (''R'') + [[S]] (S<sub>''P''</sub>) | | [[ROUTINE]] (''R'') + [[S]] (S<sub>''P''</sub>) | ||
| | | dce with functional mitochondria are in state S<sub>''P''</sub>. | ||
|- | |- | ||
| | | 3ceOmy | ||
| ''L''+S<sub>''L''</sub> | | ''L''+S<sub>''L''</sub> | ||
| [[LEAK]] (''L'') + [[S]] (S<sub>''L''</sub>) | | [[LEAK]] (''L'') + [[S]] (S<sub>''L''</sub>) | ||
| ce and | | ce and dce are in the LEAK state. | ||
|- | |- | ||
| | | 4ceU | ||
| ''E''+S<sub>''E''</sub> | | ''E''+S<sub>''E''</sub> | ||
| [[ETS]] (''E'') + [[S]] (S<sub>''E''</sub>) | | [[ETS]] (''E'') + [[S]] (S<sub>''E''</sub>) | ||
| ce and | | ce and dce are in the ETS state. | ||
|- | |- | ||
| | | 5ceRot | ||
| ROX+S<sub>''E''</sub> | | ROX+S<sub>''E''</sub> | ||
| [[ROX]]+[[S]] (S<sub>''E''</sub>) | | [[ROX]]+[[S]] (S<sub>''E''</sub>) | ||
| ce are in the ROX state, | | ce are in the ROX state, dce are in the ETS state. | ||
|- | |- | ||
| | | 6ceAma | ||
| ROX<sub>Ama</sub> | | ROX<sub>Ama</sub> | ||
| [[ROX]] | | [[ROX]] | ||
| Line 61: | Line 61: | ||
:::* ''' | :::* '''1ce;1ceS ..''' versus '''1ce;2ceOmy;3ceU;4ceS .. and respiration medium''' | ||
:::: '''Intact cells & succinate''': ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Cell Biochem Biophys]]). | :::: '''Intact cells & succinate''': ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Cell Biochem Biophys]]). | ||
Revision as of 09:18, 5 August 2017
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
Abbreviation: RLE(SD)
Reference: A Stadlmann 2006 Cell Biochem Biophys
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: RLE(SD)
- SUIT protocol pattern: complex cell coupling control protocol (ceCCP)
,3L(S),4E(S)-5ROX(S),6ROX.jpg)
Coupling control protocol
- 1ce;2ceSD;3ceOmy;4ceU;5ceRot;6ceAma
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| 1ce | ROUTINE | Intact cells (ce) | Dead cells (dce) without substrate are in a state of ROX. |
| 2ceSD | R+SP | ROUTINE (R) + S (SP) | dce with functional mitochondria are in state SP. |
| 3ceOmy | L+SL | LEAK (L) + S (SL) | ce and dce are in the LEAK state. |
| 4ceU | E+SE | ETS (E) + S (SE) | ce and dce are in the ETS state. |
| 5ceRot | ROX+SE | ROX+S (SE) | ce are in the ROX state, dce are in the ETS state. |
| 6ceAma | ROXAma | ROX |
- 1ce;1ceS .. versus 1ce;2ceOmy;3ceU;4ceS .. and respiration medium
- Intact cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).
