Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-"
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{{MitoPedia | {{MitoPedia | ||
|abbr=1R,2S,2D,3Omy,4U,5Rot,6Ama | |abbr=1R,2S,2D,3Omy,4U,5Rot,6Ama | ||
|description=[[File: | |description=[[File:1R,2S,2D,3Omy,4U,5Rot,6Ama.jpg|200px]] | ||
|info='''A''' [[Stadlmann 2006 Cell Biochem Biophys]] | |info='''A''' [[Stadlmann 2006 Cell Biochem Biophys]] | ||
}} | }} | ||
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::: '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP(S) | ::: '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP(S) | ||
::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | ::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | ||
[[File:CCP(S)02(R,L,E).jpg|right|200px]] | [[File:CCP(S)02(R,L,E).jpg|right|200px]] | ||
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|- | |- | ||
| | | 1R | ||
| [[ROUTINE]] | | [[ROUTINE]] | ||
| [[Intact cells |ce]] | | [[Intact cells |ce]] | ||
| Line 30: | Line 28: | ||
|- | |- | ||
| | | 2S,2D | ||
| ''R''+S<sub>''P''</sub> | | ''R''+S<sub>''P''</sub> | ||
| [[ROUTINE]] (''R'') + [[S]] (S<sub>''P''</sub>) | | [[ROUTINE]] (''R'') + [[S]] (S<sub>''P''</sub>) | ||
| Line 36: | Line 34: | ||
|- | |- | ||
| | | 3Omy | ||
| ''L''+S<sub>''L''</sub> | | ''L''+S<sub>''L''</sub> | ||
| [[LEAK]] (''L'') + [[S]] (S<sub>''L''</sub>) | | [[LEAK]] (''L'') + [[S]] (S<sub>''L''</sub>) | ||
| Line 42: | Line 40: | ||
|- | |- | ||
| | | 4U | ||
| ''E''+S<sub>''E''</sub> | | ''E''+S<sub>''E''</sub> | ||
| [[ETS]] (''E'') + [[S]] (S<sub>''E''</sub>) | | [[ETS]] (''E'') + [[S]] (S<sub>''E''</sub>) | ||
| Line 48: | Line 46: | ||
|- | |- | ||
| | | 5Rot | ||
| ROX+S<sub>''E''</sub> | | ROX+S<sub>''E''</sub> | ||
| [[ROX]]+[[S]] (S<sub>''E''</sub>) | | [[ROX]]+[[S]] (S<sub>''E''</sub>) | ||
| Line 54: | Line 52: | ||
|- | |- | ||
| | | 6Ama | ||
| ROX<sub>Ama</sub> | | ROX<sub>Ama</sub> | ||
| [[ROX]] | | [[ROX]] | ||
| Line 62: | Line 60: | ||
:::* ''' | :::* '''1R 1S ..''' versus '''1R 2Omy 3U 4S .. and respiration medium''' | ||
:::: '''Intac cells & succinate''': ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Cell Biochem Biophys]]). | :::: '''Intac cells & succinate''': ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types ([[Steinlechner-Maran 1997 Transplantation]]). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability ([[Stadlmann_2006_Cell Biochem Biophys]]). | ||
Revision as of 23:10, 12 March 2017
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
Abbreviation: 1R,2S,2D,3Omy,4U,5Rot,6Ama
Reference: A Stadlmann 2006 Cell Biochem Biophys
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-catg: CCP(S)
- SUIT protocol pattern: complex coupling control protocol (CCP)
Coupling control protocol
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| 1R | ROUTINE | ce | pce without substrate are in a state of ROX. |
| 2S,2D | R+SP | ROUTINE (R) + S (SP) | pce are in state SP. |
| 3Omy | L+SL | LEAK (L) + S (SL) | ce and pce are in the LEAK state. |
| 4U | E+SE | ETS (E) + S (SE) | ce and pce are in the ETS state. |
| 5Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, pce are in the ETS state. |
| 6Ama | ROXAma | ROX |
- 1R 1S .. versus 1R 2Omy 3U 4S .. and respiration medium
- Intac cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).
02(R,L,E).jpg){kind=link}