Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-"
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{{MitoPedia | {{MitoPedia | ||
|abbr=CCP_R 1SD 2Omy 3U 4Rot 5Ama | |abbr=CCP_R,1SD,2Omy,3U-4Rot,5Ama | ||
|description=[[File: | |description=[[File:CCP(S)02.jpg|200px]] | ||
|info=[[Stadlmann 2006 Cell Biochem Biophys]] | |info=[[Stadlmann 2006 Cell Biochem Biophys]] | ||
}} | }} | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=SUIT protocol | |mitopedia concept=SUIT protocol, SUIT A | ||
}} | }} | ||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics}} | |||
::: '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP(S) | ::: '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP(S) | ||
::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | ::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | ||
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::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' ''in prep.'': CCP(S)02.xlsx | ::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' ''in prep.'': CCP(S)02.xlsx | ||
[[File:CCP R | [[File:CCP(S)02(R,L,E).jpg|right|200px]] | ||
[[File:Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg|500px]] | [[File:Stadlmann 2006 Cell Biochem Biophys Fig1B-updated.jpg|500px]] | ||
== Coupling control protocol == | == Coupling control protocol == |
Revision as of 04:57, 18 September 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
Abbreviation: CCP_R,1SD,2Omy,3U-4Rot,5Ama
Reference: Stadlmann 2006 Cell Biochem Biophys
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-catg: CCP(S)
- SUIT protocol pattern: complex coupling control protocol (CCP)
- Mark names in DatLab: CCP(S)02
- DatLab-Excel template: in prep.: CCP(S)02.xlsx
Coupling control protocol
Step | Respiratory state | Pathway control | Comment |
---|---|---|---|
R | ROUTINE | ce | pce without substrate are in a state of ROX. |
1SD | R+SP | ROUTINE (R) + S (SP) | pce are in state SP. |
2Omy | L+SL | LEAK (L) + S (SL) | ce and pce are in the LEAK state. |
3U | E+SE | ETS (E) + S (SE) | ce and pce are in the ETS state. |
4Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, pce are in the ETS state. |
5Ama | ROXAma | ROX |
- R 1S .. versus R 1Omy 2U 3S .. and respiration medium
- Intac cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).