Difference between revisions of "SUIT-001 O2 ce-pce D003"
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|info='''A: RP1''' - [[SUIT-001]] | |info='''A: RP1''' - [[SUIT-001]] | ||
|application=O2 | |application=O2 | ||
|SUIT number= | |SUIT number=D003_ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd | ||
}} | }} | ||
::: [[MitoPedia: SUIT]] - '''[[SUIT reference protocol]] RP1''' | ::: [[MitoPedia: SUIT]] - '''[[SUIT reference protocol]] RP1''' | ||
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== References == | == References == | ||
{{#ask:[[Category:Publications]] [[Additional label::SUIT-1 O2 pce | {{#ask:[[Category:Publications]] [[Additional label::SUIT-1 O2 pce D003]] | ||
| ?Was published in year=Year | | ?Was published in year=Year | ||
| ?Has title=Reference | | ?Has title=Reference | ||
Revision as of 11:01, 17 January 2019
- high-resolution terminology - matching measurements at high-resolution
SUIT-001 O2 ce-pce D003
Description
Abbreviation: FNSGp(Oct,PGM)
Reference: A: RP1 - SUIT-001
SUIT number: D003_ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd
O2k-Application: O2
- MitoPedia: SUIT - SUIT reference protocol RP1
- SUIT-category: FNSGp(PGM)
- SUIT protocol pattern: orthogonal 1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp
Harmonized SUIT protocols
RP1 and RP2
SUIT RP1 
SUIT RP2 
- SUIT RP1:1PM;2D:2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd
- SUIT RP2:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
Harmonization between RP1 and RP2
RP1 and RP2 in cells
RP1 can be assessed in cells after the selective plasma membrane permeabilization. Digitonin is a mild detergent that permeabilizes plasma membranes. The optimum effective digitonin concentration for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see Digitonin). The use of intact cells allows the possibility to evaluate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, whereas after the use of digitonin we can address differentet Electron transfer-pathway states.
- SUIT RP1 in cells:ce1;1Dig;1PM;2D:2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd
- SUIT RP2 in cells:ce1;1Dig;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azdd
Harmonization between RP1 and RP2 in cells
RP1 and RP2 in PBMCs and PTLs
In PBMCs and PLTs the reference protocol RP1 is performed in the absense of Oct: SUIT 1 for PBMCs and PTLs
- SUIT RP1 for PBMCs and PLTs:1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd
- SUIT RP2:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd
Harmonization between RP1 and RP2 in PBMC and PTL cells
References
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry
