Palmitoylcarnitine: Difference between revisions
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|abbr=Pal | |abbr=Pal | ||
|description='''Palmitoylcarnitine''' is an ester derivative of [[carnitine]] (long-chain acylcarnitine) involved in the metabolism of fatty acids. Within the cell, palmitoylcarnitine is transported into the mitochondria to deliver palmitate for fatty acid oxidation and energy production. | |description='''Palmitoylcarnitine''' is an ester derivative of [[carnitine]] (long-chain acylcarnitine) involved in the metabolism of fatty acids. Within the cell, palmitoylcarnitine is transported into the mitochondria to deliver palmitate for fatty acid oxidation and energy production. | ||
|info=[[Cecatto 2023 MitoFit]] | |||
|type=Substrate ETS | |type=Substrate ETS | ||
|methods_type=Substrate ETS | |||
|topics_type=Substrate ETS | |||
}} | }} | ||
__TOC__ | __TOC__ | ||
'''Carnitine palmitoyltransferase I''' (CPT1) also known as '''carnitine acyltransferase I''' or CAT1 is a mitochondrial enzyme. CPT1 is associated with the outer mitochondrial membrane and mediates the transport of long-chain fatty acids across the membrane by binding them to carnitine. | '''Carnitine palmitoyltransferase I''' (CPT1) also known as '''carnitine acyltransferase I''' or CAT1 is a mitochondrial enzyme. CPT1 is associated with the outer mitochondrial membrane and mediates the transport of long-chain fatty acids across the membrane by binding them to carnitine. | ||
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:::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | :::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume: | ::::* Titration volume: 2 µL using a 10 µL syringe (2 mL O2k-chamber). | ||
::::* Final concentration: | ::::* Final concentration: 10 µM. We suggest testing for the optimum concentration for your sample, as palmitoylcarnitine may inhibit respiration ([[Cecatto 2023 MitoFit]]). | ||
{{MitoPedia methods|type=Substrate ETS | |||
{{MitoPedia concepts}} | |||
{{MitoPedia methods | |||
|type=Substrate ETS | |||
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{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Substrate and metabolite | |mitopedia topic=Substrate and metabolite | ||
Latest revision as of 12:04, 10 April 2024
- high-resolution terminology - matching measurements at high-resolution
Palmitoylcarnitine
Description
Palmitoylcarnitine is an ester derivative of carnitine (long-chain acylcarnitine) involved in the metabolism of fatty acids. Within the cell, palmitoylcarnitine is transported into the mitochondria to deliver palmitate for fatty acid oxidation and energy production.
Abbreviation: Pal
Reference: Cecatto 2023 MitoFit
Carnitine palmitoyltransferase I (CPT1) also known as carnitine acyltransferase I or CAT1 is a mitochondrial enzyme. CPT1 is associated with the outer mitochondrial membrane and mediates the transport of long-chain fatty acids across the membrane by binding them to carnitine.
Application in HRR
- Pal: Palmitoylcarnitine (Palmitoyl-DL-carnitine-HCl; C23H46NO4 · Cl), Sigma-Aldrich: P4509, store at -20 °C, CAS: 6865-14-1, M = 436.07 g·mol-1
- Preparation of 10 mM stock solution (dissolved in H2O):
- Weigh 8.72 mg of palmitoylcarnitine.
- Dissolve in 2 mL H2O.
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 2 µL using a 10 µL syringe (2 mL O2k-chamber).
- Final concentration: 10 µM. We suggest testing for the optimum concentration for your sample, as palmitoylcarnitine may inhibit respiration (Cecatto 2023 MitoFit).
MitoPedia topics:
Substrate and metabolite
Labels:
MitoPedia:FAT4BRAIN
