Palmitoyl-CoA: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
:::'''Pa-CoA: Palmitoyl-CoA''' (Palmitoyl-Coenzyme A lithium salt; Sigma Aldrich, P9716, 5 mg, store at -20ยฐC; M = 1004.94 gยทmol<sup>-1</sup> | ย | ||
{{Chemical_description | |||
|abbr=Pa-CoA | |||
|trivial name=Palmitoyl-CoA | |||
|complete name=Palmitoyl-Coenzyme A lithium salt | |||
|chem formula=C<sub>37</sub>H<sub>65</sub>N<sub>7</sub>O<sub>17</sub>P<sub>3</sub>S ยท xLi<sup>+</sup> | |||
|molar mass=1004.94 | |||
|vendor=Sigma-Aldrich | |||
|product number=P9716 | |||
|store at=-20 ยฐC | |||
|sensitivity= | |||
|cas=188174-64-3 | |||
|h statements= | |||
|h info= | |||
}}<!--:::'''Pa-CoA: Palmitoyl-CoA''' (Palmitoyl-Coenzyme A lithium salt; Sigma Aldrich, P9716, 5 mg, store at -20ยฐC; M = 1004.94 gยทmol<sup>-1</sup>--> | |||
ย | |||
::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): | ::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): | ||
::::# Weigh 5 mg Palmitoyl-CoA. | ::::# Weigh 5 mg Palmitoyl-CoA. | ||
Revision as of 12:14, 22 June 2021
- high-resolution terminology - matching measurements at high-resolution
Palmitoyl-CoA
Description
Palmitoyl-CoA is a coenzyme A derivative of palmitate formed by acyl-CoA synthase. In contrast to medium- and short-chain acyl-CoA, palmitoyl-CoA cannot freely diffuse into the mitochondrial matrix. Formation of palmitoylcarnitine by CPTI is necessary prior to transfer into mitochondria for further fatty acid oxidation (ฮฒ-oxidation). To study Fatty acid oxidation using Palmitoyl-CoA, Carnitine and low amount of malate is needed on mitochondrial preparations.
Application in HRR
- Pa-CoA: Palmitoyl-CoA (Palmitoyl-Coenzyme A lithium salt; C37H65N7O17P3S ยท xLi+), Sigma-Aldrich: P9716, store at -20 ยฐC, CAS: 188174-64-3, M = 1004.94 gยทmol-1
- Preparation of 10 mM stock solution (dissolved in H2O):
- Weigh 5 mg Palmitoyl-CoA.
- Add 497.5 ยตL H2O.
- Divide into 100 ยตL portions.
- Store at -20ยฐC.
- Preparation of 10 mM stock solution (dissolved in H2O):
- ยปO2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 8 ยตL 10 mM using a 25 ยตL Hamilton syringe (2 mL O2k-Chamber).
- Final concentration: 40 ยตM.
- Comments: Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type and overtitration of Palmitoyl-CoA might inhibit the respiration.
- ยปO2k manual titrations MiPNet09.12 O2k-Titrations
MitoPedia topics: Substrate and metabolite
