Palmitoyl-CoA: Difference between revisions

Revision as of 16:23, 26 April 2019

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Palmitoyl-CoA

Description

Palmitoyl-CoA is a coenzyme A derivative of palmitate formed by acyl-CoA synthase. In contrast to medium- and short-chain acyl-CoA, palmitoyl-CoA cannot freely diffuse into the mitochondrial matrix. Formation of palmitoylcarnitine by CPTI is necessary prior to transfer into mitochondria for further fatty acid oxidation (β-oxidation). To study Fatty acid oxidation using Palmitoyl-CoA, Carnitine and low amount of malate is needed on mitochondrial preparations.

MitoPedia topics: Substrate and metabolite

Application in HRR

Pa-CoA: Palmitoyl-CoA (Palmitoyl-Coenzym A lithium salt; Sigma, P9716, 5 mg, store at -20°C; FW=1004.94) Preparation of 10 mM stock solution (dissolved in H₂O):

Weigh 5 mg Palmitoyl-CoA.
Add 497.5 µL H₂O.
Divide into 100 µL portions.
Store at -20°C.

O2k manual titrations

Titration volume: 8 µL 10 mM using a 25 µL Hamilton syringe (2 mL O2k-Chamber).
Final concentration: 40 µM.
Comments: Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type and overtitration of Palmitoyl-CoA might inhibit the respiration.

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 {{MitoPedia
-|description='''Palmitoyl-CoA''' is a coenzyme A derivative of palmitate formed by acyl-CoA synthase. In contrast to medium- and short-chain acyl-CoA, palmitoyl-CoA cannot freely diffuse into the mitochondrial matrix. Formation of palmitoylcarnitine by CPTI is necessary prior to transfer into mitochondria for further fatty acid oxidation (β-oxidation).
+|description='''Palmitoyl-CoA''' is a coenzyme A derivative of palmitate formed by acyl-CoA synthase. In contrast to medium- and short-chain acyl-CoA, palmitoyl-CoA cannot freely diffuse into the mitochondrial matrix. Formation of palmitoylcarnitine by CPTI is necessary prior to transfer into mitochondria for further fatty acid oxidation (β-oxidation). To study [[Fatty acid oxidation]] using Palmitoyl-CoA, [[Carnitine]] and low amount of malate is needed on mitochondrial preparations.
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 }}
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+== Application in [[HRR]] ==
+'''Pa-CoA: Palmitoyl-CoA''' (Palmitoyl-Coenzym A lithium salt; Sigma, P9716, 5 mg, store at -20°C; FW=1004.94)
+'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
+::::# Weigh 5 mg Palmitoyl-CoA.
+::::# Add 497.5 µL H<sub>2</sub>O.
+::::# Divide into 100 µL portions.
+::::# Store at -20°C.
+'''O2k manual titrations'''
+::::* Titration volume: 8 µL 10 mM using a 25 µL Hamilton syringe (2 mL O2k-Chamber).
+::::* Final concentration: 40 µM.
+::::* Comments: Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type and overtitration of Palmitoyl-CoA might inhibit the respiration.